Andreason Sharon A, Lahey Zachary, Zhao Dongyan, Mejia-Guerra Katherine, Williams Livy H, Sheehan Moira, Simmons Alvin M, Wadl Phillip A
United States Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy., Charleston, SC 29414, United States of America.
Breeding Insight, Cornell University, 525 Tower Rd., Ithaca, NY 14853, United States of America.
Data Brief. 2023 Jul 21;49:109432. doi: 10.1016/j.dib.2023.109432. eCollection 2023 Aug.
The sweetpotato weevil, (Summers) (Coleoptera: Brentidae), is one of the most destructive pests of sweetpotato worldwide. Genomic analyses of sweetpotato weevils can provide insights into their genetic diversity, population structure, and dispersal as well as provide information to support management strategies. Adult sweetpotato weevils were collected by various methods from L. (sweetpotato) or L. (red morning glory) in the U.S. states of Georgia, Hawaii, South Carolina, and Texas. Genomic DNA was extracted from individual weevil specimens and sequenced using Illumina NovaSeq. A total of 181 GB of 150 base pair (bp) paired-end reads were generated for 40 specimens. Mitochondrial genomes were assembled for each specimen via reference mapping and annotated using Geneious Prime. Full mitochondrial genome sequences range from 17,141 to 17,152 bp with an average GC content of 21.8% and average coverage of 3307 × . A maximum likelihood phylogenetic analysis considering the mitochondrial protein coding genes is provided. Mitochondrial genomes and assembled reads are deposited in NCBI GenBank, providing 40 mitogenomes of collected in the U.S.
甘薯象甲（Summers）（鞘翅目：蛛甲科）是全球甘薯最具毁灭性的害虫之一。对甘薯象甲的基因组分析可以深入了解其遗传多样性、种群结构和扩散情况，并为支持管理策略提供信息。通过多种方法从美国佐治亚州、夏威夷州、南卡罗来纳州和得克萨斯州的甘薯（Ipomoea batatas L.）或裂叶牵牛（Ipomoea hederacea Jacq. L.）上采集成年甘薯象甲。从单个象甲标本中提取基因组DNA，并使用Illumina NovaSeq进行测序。为40个标本生成了总共181GB的150碱基对（bp）双端读数。通过参考图谱为每个标本组装线粒体基因组，并使用Geneious Prime进行注释。完整的线粒体基因组序列长度在17,141至17,152bp之间，平均GC含量为21.8%，平均覆盖度为3307×。提供了考虑线粒体蛋白质编码基因的最大似然系统发育分析。线粒体基因组和组装读数已存入NCBI GenBank，提供了在美国采集的40个甘薯象甲的线粒体基因组。
