Behnam Shayesteh, Zavareh Saeed, Salehnia Mojdeh
Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
School of Biology, Damghan University, Damghan, Iran.
J Family Reprod Health. 2023 Jun;17(2):65-72. doi: 10.18502/jfrh.v17i2.12868.
Oxidative stress affects the development of ovarian follicles during culture thus applying an antioxidant is necessary. The aim of this study was to investigate the effect of coenzyme Q (CoQ) on the expression of apoptosis-related genes of mouse ovaries during culture.
The immature mouse ovaries were cultured in the presence of 50 μM CoQ for 7 days. Histological examinations were performed and the 17 beta-estradiol concentration was measured on the seventh day of culture. The relative expression of 3, , , and 2 genes were investigated by real-time RT-PCR.
The rates of normal follicles in the presence of CoQ was significantly increased compared to the control group. Also, in CoQ-trated group a significant increase in the level of 17 beta-estradiol was seen compared to the control group. The mRNA expression of anti-apoptotic gene 2 was significantly increased while the expression of pro-apoptotic genes (3, and ) significantly declined in CoQ treated group compared to those of control group.
The supplementation of the ovarian culture media with CoQ improved the follicular development through alteration in expression of apoptosis-related genes and stimulated the production of estradiol.
氧化应激会影响培养过程中卵巢卵泡的发育，因此应用抗氧化剂是必要的。本研究的目的是探讨辅酶Q（CoQ）对培养过程中小鼠卵巢凋亡相关基因表达的影响。
将未成熟小鼠卵巢在50μM CoQ存在下培养7天。进行组织学检查，并在培养的第7天测量17β-雌二醇浓度。通过实时RT-PCR研究3、、和2基因的相对表达。
与对照组相比，CoQ存在下正常卵泡的比例显著增加。此外，与对照组相比，CoQ处理组中17β-雌二醇水平显著升高。与对照组相比，CoQ处理组抗凋亡基因2的mRNA表达显著增加，而促凋亡基因（3、和）的表达显著下降。
在卵巢培养基中添加CoQ可通过改变凋亡相关基因的表达改善卵泡发育，并刺激雌二醇的产生。
