College of Animal Science, Yangtze University, Jingzhou, 434025, Hubei, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Theriogenology. 2021 Jan 1;159:77-86. doi: 10.1016/j.theriogenology.2020.10.009. Epub 2020 Oct 9.
Coenzyme Q10 (CoQ10) is essential to many fundamental biological processes. However, the effect of CoQ10 on meiotic maturation of pig oocytes still remains elusive. In the present study we aimed to understand the effects of CoQ10 on porcine oocyte maturation, by supplementing different concentrations of CoQ10 (25, 50 and 100 μM) into the maturation medium. We showed that CoQ10 at 50 μM had better capacity to promote the nuclear maturation of pig oocytes derived from both small and large antral follicles. Though the cleavage and blastocyst rates of parthenotes stayed stable, 50 μM CoQ10 treatment could accelerate the development of parthenotes to blastocyst stage, and increase the average cell number of blastocyst. For cumulus-oocyte complexes from large antral follicles categorized by the brilliant cresyl blue (BCB) test, 50 μM CoQ10 treatment could specifically promote the nuclear maturation of poor-quality oocytes in the BCB-negative group. Mitochondrial function of oocytes treated by 50 μM CoQ10 could be boosted, through increasing the levels of mitochondrial membrane potential, ATP production and CoQ6, and changing the pattern of mitochondrial distribution as well. Moreover, 50 μM CoQ10 treatment suppressed the level of reactive oxygen species and reduced the percentage of oocytes with early apoptosis signal. Taken together, CoQ10 could improve the meiotic maturation of pig oocytes, especially for poor-quality oocytes, mainly through enhancing mitochondrial function and suppressing oxidative stress to reduce apoptosis.
辅酶 Q10（CoQ10）对许多基本的生物过程至关重要。然而，CoQ10 对猪卵母细胞减数分裂成熟的影响仍不清楚。在本研究中，我们旨在通过在成熟培养基中添加不同浓度的 CoQ10（25、50 和 100μM）来了解 CoQ10 对猪卵母细胞成熟的影响。我们表明，50μM 的 CoQ10 具有更好的促进小和大腔卵泡来源的猪卵母细胞核成熟的能力。虽然孤雌激活胚胎的卵裂和囊胚率保持稳定，但 50μM CoQ10 处理可以加速孤雌激活胚胎向囊胚阶段的发育，并增加囊胚的平均细胞数。对于根据亮甲酚蓝（BCB）试验分类的大腔卵泡的卵丘-卵母细胞复合物，50μM CoQ10 处理可以特异性促进 BCB 阴性组中质量差的卵母细胞核成熟。通过增加线粒体膜电位、ATP 产生和 CoQ6 的水平以及改变线粒体分布模式，可以增强经 50μM CoQ10 处理的卵母细胞的线粒体功能。此外，50μM CoQ10 处理可抑制活性氧水平并降低具有早期凋亡信号的卵母细胞的百分比。总之，CoQ10 可以改善猪卵母细胞的减数分裂成熟，特别是对于质量差的卵母细胞，主要通过增强线粒体功能和抑制氧化应激来减少凋亡。
