缪勒氏管抑制物质的牛和人类基因的分离以及人类基因在动物细胞中的表达。

Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells.

作者信息

Cate R L, Mattaliano R J, Hession C, Tizard R, Farber N M, Cheung A, Ninfa E G, Frey A Z, Gash D J, Chow E P

出版信息

Cell. 1986 Jun 6;45(5):685-98. doi: 10.1016/0092-8674(86)90783-x.

Abstract

We have isolated the bovine and human genes for Müllerian inhibiting substance (MIS), a testicular glycoprotein that causes regression of the Müllerian duct during development of the male embryo. The mRNA sequence of bovine MIS, determined from an analysis of cDNA and genomic clones, codes for a protein of 575 amino acids containing a 24 amino acid leader peptide. The human gene has five exons that code for a protein of 560 amino acids. A comparison of the bovine and human MIS proteins reveals a highly conserved C-terminal domain that shows marked homology with human transforming growth factor-beta and the beta chain of porcine inhibin. Animal cells transfected with the human gene secrete biologically active MIS, which causes regression of the rat Müllerian duct in vitro.

摘要

我们已经分离出了缪勒管抑制物质（MIS）的牛和人类基因，MIS是一种睾丸糖蛋白，在雄性胚胎发育过程中会导致缪勒管退化。通过对cDNA和基因组克隆的分析确定的牛MIS的mRNA序列编码一种由575个氨基酸组成的蛋白质，其中包含一个24个氨基酸的前导肽。人类基因有五个外显子，编码一种由560个氨基酸组成的蛋白质。牛和人类MIS蛋白的比较显示，其C端结构域高度保守，与人类转化生长因子-β和猪抑制素的β链具有显著同源性。用人类基因转染的动物细胞分泌具有生物活性的MIS，它在体外会导致大鼠缪勒管退化。