Department of Medicine, Graduate School, Chung-Ang University, Seoul, South Korea.
Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
Lasers Surg Med. 2023 Oct;55(8):758-768. doi: 10.1002/lsm.23707. Epub 2023 Aug 7.
A novel dual-length microneedle radiofrequency (DLMR) device has been developed to achieve full-thickness skin rejuvenation by stimulating the papillary and reticular dermis simultaneously. This device's dual-level targeting concept need to be validated on human skin, although its clinical efficacy has been demonstrated in a previous study.
This study evaluated the dual-depth targeting capability and the ability to induce rejuvenation in each layer of vertical skin anatomy, that is, the epidermis, papillary dermis, and reticular dermis, using full-thickness human facial skin samples.
Human facial skin samples were obtained from 13 Asian patients who had facelift surgery. To validate the dual-depth targeting concept, DMLR-treated skin samples were analyzed using a digital microscope, thermal imaging, and hematoloxylin and eosin (H&E) staining immediately after DLMR application. On samples stained with H&E, Masson's tricrome, and Verhoeff-Van Gieson, histological observation and morphometric analysis were performed. Total collagen assay (TCA) and quantitative real-time polymerase chain reaction (qPCR) were used to assess changes in total collagen content and mRNA expression levels of collagen types I/III and vimentin, respectively.
The DLMR device successfully induced thermal stimulation in the papillary and reticular dermis. The thickness, stacks, and dermal-epidermal junction convolution of the epidermis treated with DLMR were significantly increased. Collagen bundles in the dermis treated with DLMR exhibited a notable increase in thickness, density, and horizontal alignment. Dermal collagen levels were significantly higher in the morphometric and TCA data, as well as in the qPCR data for dermal matrix proteins.
Our DLMR device independently and precisely targeted the papillary and reticular dermis, and it appears to be an effective modality for implementing full-thickness rejuvenation.
一种新型双长度微针射频（DLMR）设备已被开发出来，通过同时刺激乳头层和网状真皮来实现全层皮肤年轻化。该设备的双层次靶向概念需要在人体皮肤上进行验证，尽管其临床疗效已在先前的研究中得到证实。
本研究评估了双深度靶向能力以及在全厚人体面部皮肤样本中诱导每个垂直皮肤解剖层（表皮、乳头真皮和网状真皮）年轻化的能力。
从接受面部提升手术的 13 名亚洲患者中获得面部皮肤样本。为了验证双深度靶向概念，在 DLMR 应用后立即使用数字显微镜、热成像和苏木精和伊红（H&E）染色分析 DMLR 处理的皮肤样本。在 H&E、Masson 三色和 Verhoeff-Van Gieson 染色的样本上进行组织学观察和形态计量分析。总胶原测定（TCA）和定量实时聚合酶链反应（qPCR）分别用于评估总胶原含量和 I/III 型胶原和波形蛋白的 mRNA 表达水平的变化。
DLMR 设备成功地在乳头层和网状真皮中引起了热刺激。经 DLMR 处理的表皮的厚度、层和真皮-表皮交界处的卷曲明显增加。经 DLMR 处理的真皮中的胶原束在厚度、密度和水平排列方面表现出显著增加。形态计量和 TCA 数据以及真皮基质蛋白的 qPCR 数据均显示真皮胶原水平显著升高。
我们的 DLMR 设备独立且精确地靶向乳头层和网状真皮，似乎是实现全层年轻化的有效手段。
