H.P. State Pollution Control Board, Regional Laboratory Paonta Sahib, Paonta Sahib, India.
Department of Basic Sciences (Microbiology Section), Dr. Y.S. Parmar, University of Horticulture and Forestry Nauni, Solan, India.
Nat Prod Res. 2024 Aug;38(16):2844-2852. doi: 10.1080/14786419.2023.2244135. Epub 2023 Aug 7.
Bacteriocin producing strain TK3, was isolated from ''- a flour prepared from seeds of Indian Horse Chestnut (). Bacterial strain TK3 identified by morphological, biochemical techniques followed by 16S rRNA gene sequencing. The 16S rRNA sequence of bacteriocin producer was deposited in NCBI GenBank under accession no. KP861913.1. Bacteriocin of TK3 showed strong antagonistic activity against food spoiling/pathogenic bacteria viz. and Bacteriocin production by TK3 was enhanced by optimizing production time, pH of the medium, inoculum size and incubation temperature. Maximum bacteriocin activity (6000 AU/ml) was recorded/obtained in basal salt medium of pH 5.5 with an inoculum size of 1.5 OD at 10% and incubation period of 24h at 35 C. The bacteriocin was purified by single step gel exclusion chromatography. Molecular weight of active bacteriocin from TK3 was found to be 6 kDa according to SDS PAGE. The molecular mass of purified bacteriocin was confirmed as 5953.89 Da by MALDI TOF analysis. The purified bacteriocin was found desirable/suitable for food preservation as it showed wide spectrum of antimicrobial activity, resistance to high temperature, wide pH range and sensitivity to proteolytic enzymes thus, making it safe for human consumption.
产细菌素菌株 TK3 是从印度马栗（）种子制成的面粉中分离出来的。通过形态学、生化技术以及 16S rRNA 基因测序对细菌菌株 TK3 进行了鉴定。细菌素产生菌的 16S rRNA 序列已在 NCBI GenBank 中注册，登录号为 KP861913.1。TK3 的细菌素对食品腐败/病原菌（即 和 ）表现出强烈的拮抗活性。通过优化生产时间、培养基 pH 值、接种量和培养温度，可提高 TK3 的细菌素产量。在 pH 为 5.5 的基础盐培养基中，以 10%的接种量和 35°C 下 24 小时的孵育期，可获得最大的细菌素活性（6000 AU/ml）。通过单步凝胶排阻层析对细菌素进行了纯化。根据 SDS PAGE，从 TK3 中分离出的活性细菌素的分子量为 6 kDa。通过 MALDI TOF 分析，纯化细菌素的分子量确认为 5953.89 Da。由于该纯化细菌素具有广谱抗菌活性、耐高温、宽 pH 范围和对蛋白水解酶的敏感性，因此可用于食品保鲜，对人体安全。
