Yang Cairong, Yang Jiezhi, Zhou Yueju, Ou Yi, Wang ZhiMeng, Qi Weiliang, Huang Renwei, Chai Songyue, Yang Hongling, Zhou Yongfang, Liu Songqing
College of Chemistry and Life Science, Chengdu Normal University, Chengdu, Sichuan, China.
Sichuan Provincial Forestry and Grassland Key Laboratory of Biodiversity Conservation and Sustainable Community Development in Giant Panda National Park, Chengdu, Sichuan, China.
Front Microbiol. 2025 May 20;16:1596093. doi: 10.3389/fmicb.2025.1596093. eCollection 2025.
, as an edible delicious mushroom, the storage time and quality affect its economic value and industrial development. In the present study, was sealed and packaged in PE self-sealing bags and stored at a storage temperature of 4°C, 90% humidity. The physiological and biochemical indexes of were measured and the bacterial community on the surface of fruit was determined. Soluble protein rapidly decreased in the first 5 days and then stabilized. Superoxide anions and malondialdehyde, showed dynamic fluctuations. Antioxidant enzymes SOD and CAT exhibited variable activities, while POD remained stable. The activity of polyphenol oxidase ascended, while the total phenolic content initially dropped and subsequently registered a marginal increase. In tandem, the ascorbic acid (VC) content underwent a persistent decrease. Cell wall related enzyme displayed distinct temporal activity patterns. Analysis of bacterial diversity showed that Proteobacteria was the dominant phylum and the genus was the dominant bacterial genus in the storage process of . Alpha diversity analysis showed that with the extension of storage time, the bacterial diversity on the surface of the body gradually decreased. Correlation Network Analysis showed that genera , , and s were the most closely related bacterial genera to other bacteria, occupying a dominant position in the entire bacterial community. WGCNA showed that Altererythrobacter and Brevibacilluswere might improve the storage quality of . WGCNA also showed that and might disrupt the cell wall structure of . This study shed light on the understanding of the physiological indicators changes and bacterial community diversity on the surface of fruit during storage. It also provided analysis of the correlation between physiological indicator changes and bacterial community diversity on the surface of fruit, which could provide some support for scientific storage of .
作为一种可食用的美味蘑菇,其储存时间和品质会影响其经济价值和产业发展。在本研究中,将其密封包装在PE自封袋中,并在4°C、湿度90%的储存温度下储存。测定了其生理生化指标,并确定了果实表面的细菌群落。可溶性蛋白在前5天迅速下降,然后稳定下来。超氧阴离子和丙二醛呈现动态波动。抗氧化酶SOD和CAT表现出不同的活性,而POD保持稳定。多酚氧化酶的活性上升,而总酚含量最初下降,随后略有增加。同时,抗坏血酸(VC)含量持续下降。细胞壁相关酶表现出明显的时间活性模式。细菌多样性分析表明,变形菌门是优势菌门,在其储存过程中,属是优势细菌属。α多样性分析表明,随着储存时间的延长,其体表细菌多样性逐渐降低。相关网络分析表明,属、属和属是与其他细菌关系最密切的细菌属,在整个细菌群落中占据主导地位。加权基因共表达网络分析(WGCNA)表明,交替赤杆菌属和短短芽孢杆菌属可能改善其储存品质。WGCNA还表明,属和属可能破坏其细胞壁结构。本研究有助于了解其果实储存期间生理指标变化和表面细菌群落多样性。还提供了其果实表面生理指标变化与细菌群落多样性之间的相关性分析,可为其科学储存提供一定支持。