与乙二胺四乙酸（EDTA）相比，使用肝素进行采血会导致4小时时单核细胞分布宽度在不依赖炎性小体的情况下增加。 - Suppr

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Blood collection in heparin vs. EDTA results in an inflammasome-independent increase in monocyte distribution width at 4 h.

作者信息

Eisinger Gregory J, Osman Wissam, Prather Evan R, Julian Mark W, Gavrilin Mikhail A, Crouser Elliott D, Wewers Mark D

机构信息

Pulmonary/Critical Care and Emergency Medicine, The Ohio State University, Wexner Medical Center, Columbus, OH, USA.

The Ohio State University, Wexner Medical Center, Columbus, OH, USA.

出版信息

Clin Chem Lab Med. 2023 Aug 7;62(2):e26-e28. doi: 10.1515/cclm-2023-0767. Print 2024 Jan 26.

DOI:10.1515/cclm-2023-0767

PMID:37548464

Abstract

摘要

相似文献

Blood collection in heparin vs. EDTA results in an inflammasome-independent increase in monocyte distribution width at 4 h.与乙二胺四乙酸（EDTA）相比，使用肝素进行采血会导致4小时时单核细胞分布宽度在不依赖炎性小体的情况下增加。

Clin Chem Lab Med. 2023 Aug 7;62(2):e26-e28. doi: 10.1515/cclm-2023-0767. Print 2024 Jan 26.

Tumor necrosis factor measurement and use of different anticoagulants: possible interference in plasma samples and supernatants from endotoxin-stimulated monocytes.肿瘤坏死因子的测定及不同抗凝剂的使用：对内毒素刺激单核细胞的血浆样本和上清液可能产生的干扰

Inflamm Res. 1997 Sep;46(9):342-7. doi: 10.1007/s000110050199.

Influence of five different anticoagulants on human blood monocyte isolation and functional activities.五种不同抗凝剂对人血单核细胞分离及功能活性的影响。

Acta Pathol Microbiol Immunol Scand C. 1985 Apr;93(2):49-52. doi: 10.1111/j.1699-0463.1985.tb02921.x.

The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes.不同抗凝剂和样本制备方法对牛单核细胞和多形核中性粒细胞上mCD14测量的影响。

BMC Res Notes. 2012 Feb 14;5:93. doi: 10.1186/1756-0500-5-93.

Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood.在全血单细胞水平评估乙二胺四乙酸（EDTA）和肝素对脂多糖结合及细胞活化的影响。

Cytometry. 2002 Feb 15;50(1):14-8.

Modulation of blood cell activation by four commonly used anticoagulants.四种常用抗凝剂对血细胞活化的调节作用。

Thromb Haemost. 1997 Apr;77(4):690-6.

Monocyte antigen-presenting capacity to iNKT cells is influenced by the blood collection conditions.单核细胞向不变自然杀伤T细胞呈递抗原的能力受采血条件影响。

J Immunol Methods. 2023 Feb;513:113426. doi: 10.1016/j.jim.2023.113426. Epub 2023 Jan 11.

Inflammasome Activation in an In Vitro Sepsis Model Recapitulates Increased Monocyte Distribution Width Seen in Patients With Sepsis.体外脓毒症模型中的炎性小体激活再现了脓毒症患者中观察到的单核细胞分布宽度增加的情况。

Crit Care Explor. 2022 Feb 1;4(2):e0631. doi: 10.1097/CCE.0000000000000631. eCollection 2022 Feb.

COMPARING THE EFFECTS OF LITHIUM HEPARIN AND DIPOTASSIUM ETHYLENEDIAMINETETRAACETIC ACID ON HEMATOLOGIC VALUES IN EASTERN BOX TURTLES ().比较肝素锂和乙二胺四乙酸二钾对东部箱龟血液学值的影响（）。

J Zoo Wildl Med. 2021 Jan;51(4):999-1006. doi: 10.1638/2020-0109.

Analysis of genome-wide 5-hydroxymethylation of blood samples stored in different anticoagulants: opportunities for the expansion of clinical resources for epigenetic research.分析不同抗凝剂保存的血样中的全基因组 5-羟甲基化：拓展表观遗传学研究临床资源的机会。

Epigenetics. 2023 Dec;18(1):2271692. doi: 10.1080/15592294.2023.2271692. Epub 2023 Oct 29.

本文引用的文献

Monocyte distribution width (MDW) as a screening tool for early detecting sepsis: a systematic review and meta-analysis.单核细胞分布宽度（MDW）作为早期检测脓毒症的筛查工具：系统评价和荟萃分析。

Clin Chem Lab Med. 2022 Feb 15;60(5):786-792. doi: 10.1515/cclm-2021-1331. Print 2022 Apr 26.

Crit Care Explor. 2022 Feb 1;4(2):e0631. doi: 10.1097/CCE.0000000000000631. eCollection 2022 Feb.

Monocyte distribution width (MDW) as a screening tool for sepsis in the Emergency Department.单核细胞分布宽度（MDW）作为急诊科脓毒症的筛查工具。

Clin Chem Lab Med. 2020 Oct 25;58(11):1951-1957. doi: 10.1515/cclm-2020-0417.

Global, regional, and national sepsis incidence and mortality, 1990-2017: analysis for the Global Burden of Disease Study.全球、地区和国家脓毒症发病率和死亡率，1990-2017 年：全球疾病负担研究分析。

Lancet. 2020 Jan 18;395(10219):200-211. doi: 10.1016/S0140-6736(19)32989-7.

Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis.比较单核细胞分布宽度（MDW）和降钙素原对脓毒症的早期识别。

PLoS One. 2020 Jan 10;15(1):e0227300. doi: 10.1371/journal.pone.0227300. eCollection 2020.

ED Door-to-Antibiotic Time and Long-term Mortality in Sepsis.ED 门到抗生素时间与脓毒症的长期死亡率。

Chest. 2019 May;155(5):938-946. doi: 10.1016/j.chest.2019.02.008. Epub 2019 Feb 16.

Old and new applications of non-anticoagulant heparin.非抗凝肝素的新旧应用

Int J Cardiol. 2016 Jun;212 Suppl 1:S14-21. doi: 10.1016/S0167-5273(16)12004-2.

Heparin and EDTA anticoagulants differentially affect the plasma cytokine levels in humans.肝素和 EDTA 抗凝剂对人体血浆细胞因子水平有不同的影响。

Scand J Clin Lab Invest. 2013 Aug;73(5):452-5. doi: 10.3109/00365513.2013.798869. Epub 2013 Jun 17.

Heparin and EDTA as anticoagulant differentially affect cytokine mRNA level of cultured porcine blood cells.肝素和乙二胺四乙酸作为抗凝剂对培养的猪血细胞的细胞因子mRNA水平有不同影响。

J Immunol Methods. 2007 Jul 31;324(1-2):38-47. doi: 10.1016/j.jim.2007.04.012. Epub 2007 May 24.

The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes.乙二胺四乙酸（EDTA）作为用于诊断目的的体外抗凝剂的作用。

Clin Chem Lab Med. 2007;45(5):565-76. doi: 10.1515/CCLM.2007.110.

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Blood collection in heparin vs. EDTA results in an inflammasome-independent increase in monocyte distribution width at 4 h.

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Blood collection in heparin vs. EDTA results in an inflammasome-independent increase in monocyte distribution width at 4 h.