五种不同抗凝剂对人血单核细胞分离及功能活性的影响。

Influence of five different anticoagulants on human blood monocyte isolation and functional activities.

作者信息

Nielsen H

出版信息

Acta Pathol Microbiol Immunol Scand C. 1985 Apr;93(2):49-52. doi: 10.1111/j.1699-0463.1985.tb02921.x.

DOI:10.1111/j.1699-0463.1985.tb02921.x

Abstract

The recovery of blood monocytes after centrifugation with metrizoate/polysucrose was high with EDTA (97.0%) and heparin (91.6%) as anticoagulants, moderate with EGTA and citrate, but low with oxalate (52.3%; P less than 0.05 compared with heparin). There were no differences in viability, spontaneous migration, chemotaxis, or phagocytosis and killing of Candida albicans by the monocytes isolated from blood anticoagulated with heparin, citrate, oxalate, EDTA, or EGTA. However, heparin in concentrations above 20 IU/ml decreased the spontaneous migration and chemotaxis. It is concluded, that EDTA and heparin below 20 IU/ml are the most satisfactory anticoagulants for the functional study of blood monocytes isolated by metrizoate/polysucrose density centrifugation.

摘要

使用泛影葡胺/聚蔗糖离心后，以乙二胺四乙酸（EDTA，回收率97.0%）和肝素（91.6%）作为抗凝剂时，血液单核细胞的回收率较高；以乙二醇双四乙酸（EGTA）和柠檬酸盐作为抗凝剂时，回收率中等；而以草酸盐作为抗凝剂时，回收率较低（52.3%；与肝素相比，P<0.05）。从用肝素、柠檬酸盐、草酸盐、EDTA或EGTA抗凝的血液中分离出的单核细胞，在活力、自发迁移、趋化性或对白色念珠菌的吞噬和杀伤能力方面没有差异。然而，浓度高于20国际单位/毫升的肝素会降低自发迁移和趋化性。得出的结论是，对于通过泛影葡胺/聚蔗糖密度离心分离的血液单核细胞进行功能研究而言，20国际单位/毫升以下的EDTA和肝素是最理想的抗凝剂。