Research Unit VEG-i-TEC, Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, Kortrijk, Belgium.
Compr Rev Food Sci Food Saf. 2023 Sep;22(5):3910-3950. doi: 10.1111/1541-4337.13219. Epub 2023 Aug 7.
In 2023, Listeria monocytogenes persistence remains a problem in the food business. A profound understanding of how this pathogen persists may lead to better aimed intervention/prevention strategies. The lack of a uniform definition of persistence makes the comparison between studies complex. Harborage sites offer protection against adverse environmental conditions and form the ideal habitat for the formation of biofilms, one of the major persistence strategies. A retarded growth rate, disinfectant resistance/tolerance, desiccation resistance/tolerance, and protozoan protection complete the list of persistence strategies for Listeria monocytogenes and can occur on themselves or in combination with biofilms. Based on the discussed persistence strategies, intervention strategies are proposed. By enhancing the focus on four precaution principles (cleaning and disinfection, infrastructure/hygienic design, technical maintenance, and work methodology) as mentioned in Regulation (EC) No. 852/2004, the risk of persistence can be decreased. All of the intervention strategies result in obtaining and maintaining a good general hygiene status throughout the establishment at all levels ranging from separate equipment to the entire building.
2023 年，李斯特菌的持续存在仍然是食品行业的一个问题。深入了解这种病原体如何持续存在，可能会导致更有针对性的干预/预防策略。缺乏对持久性的统一定义，使得研究之间的比较变得复杂。藏身之处为应对不利的环境条件提供了保护，并为生物膜的形成提供了理想的栖息地，这是主要的持久性策略之一。生长缓慢、消毒剂抗性/耐受性、干燥耐受性/耐受性以及原生动物保护构成了李斯特菌持续存在策略的完整列表，并且可以单独发生或与生物膜结合发生。基于讨论的持久性策略，提出了干预策略。通过加强对法规 (EC) No. 852/2004 中提到的四项预防原则（清洁和消毒、基础设施/卫生设计、技术维护和工作方法）的重视，可以降低持续存在的风险。所有干预策略都可以在整个设施的各个层面（从单独的设备到整个建筑物）获得并保持良好的一般卫生状况。
