Rotta Isabela Sguilla, Rezende Sthefânia Dalva da Cunha, Perini Hugo Felix, da Silva Marcos Vinicius, de Almeida Felipe Alves, Pinto Uelinton Manoel, Ferreira Machado Alessandra Barbosa, Paiva Aline Dias
Department of Microbiology, Immunology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, Brazil.
Department of Education in Science, Mathematics and Technology, Federal University of Triângulo Mineiro, Uberaba, Brazil.
Front Microbiol. 2025 Jun 18;16:1601203. doi: 10.3389/fmicb.2025.1601203. eCollection 2025.
UFTM 2.6.1, isolated from unpasteurized milk, is a potentially probiotic strain exhibiting desirable properties previously demonstrated in vitro, along with a confirmed safe and promising genetic profile based on whole-genome analysis. Due to the limited research on the ability of species to synthesize compounds with anti-quorum sensing activity, this study aimed to investigate the potential of UFTM 2.6.1 to disrupt quorum sensing (QS) signaling and attenuate the virulence of , an important foodborne pathogen responsible for the zoonotic disease listeriosis.
The effects of cell-free supernatant (-CFS) were evaluated on the growth, biofilm formation, motility, and expression of QS- and virulence-related genes in .
-CFS exhibited bacteriostatic activity against L. monocytogenes strains isolated from food and food processing environments ( = 21). Additionally, it consistently reduced biofilm formation and swarming motility, two well-known QS-regulated phenotypes in . Exposure to -CFS (0.25x MIC; 7.81 mg/mL), at 28°C for 24 h, significantly downregulated the relative expression of the genes , and , whereas the genes , and were upregulated.
This study represents the first report demonstrating the production of compounds by W. paramesenteroides aimed at disrupting the QS system of , offering novel insights into alternative approaches to attenuate pathogen virulence without relying on traditional antimicrobials.
从未经巴氏杀菌的牛奶中分离出的UFTM 2.6.1是一种潜在的益生菌菌株，具有先前在体外已证明的理想特性，并且基于全基因组分析具有经确认的安全且有前景的基因图谱。由于对该物种合成具有抗群体感应活性化合物能力的研究有限，本研究旨在探究UFTM 2.6.1破坏群体感应（QS）信号传导并减弱单增李斯特菌毒力的潜力，单增李斯特菌是一种导致人畜共患病李斯特菌病的重要食源性病原体。
评估了UFTM 2.6.1无细胞上清液（-CFS）对单增李斯特菌的生长、生物膜形成、运动性以及QS和毒力相关基因表达的影响。
-CFS对从食品和食品加工环境中分离出的单增李斯特菌菌株（n = 21）表现出抑菌活性。此外，它持续减少生物膜形成和群游运动性，这是单增李斯特菌中两种众所周知的受QS调控的表型。在28°C下暴露于-CFS（0.25x MIC；7.81 mg/mL）24小时，显著下调了prfA、plcA和hlyA基因的相对表达，而上调了sigB、lmo0737和lmo2655基因的表达。
本研究首次报道了副肠系膜魏斯氏菌产生旨在破坏单增李斯特菌QS系统的化合物，为在不依赖传统抗菌药物的情况下减弱病原体毒力的替代方法提供了新见解。
