Department of Chemistry, Polymer Synthesis and Analysis Laboratory, Çanakkale Onsekiz Mart University, Çanakkale, 17020, Turkey.
J Fluoresc. 2023 Sep;33(5):2105-2117. doi: 10.1007/s10895-023-03371-4. Epub 2023 Aug 7.
An oligomeric nanoparticle was obtained through an enzyme-catalyzed oxidation reaction using Horse Radish Peroxidase (HRP) with the 2,3-diaminopyridine (DAP) compound as the starting material. The structural characterizations of the synthesized oligomeric nanoparticles [[oligo(DAP)]] were performed with H-NMR and FT-IR. The surface features were determined by scanning electron microscopy. The optical properties were determined by UV-Vis and fluorescence spectra. It was found that the obtained oligomeric nanoparticles had a different fluorescent character with 15.90% quantum yield from the monomer and emitted green color at 485 nm when excited with light at a wavelength of 420 nm. The electrochemical band gap of the oligomeric nanoparticles, whose electrochemical character was investigated by cyclic voltammetry, was calculated as 2.09 eV. The thermal stability of the oligomeric material was determined from the mass loss against increasing temperature. The monomer exhibited greater thermal stability in comparison to the polymer, both in terms of the temperature at which the maximum mass loss occurred and the residual amount after heating concluded.
通过使用辣根过氧化物酶（HRP）作为催化剂，以 2,3-二氨基吡啶（DAP）化合物为起始原料，进行酶催化氧化反应，得到了一种寡聚纳米粒子。通过 H-NMR 和 FT-IR 对合成的寡聚纳米粒子 [[寡聚（DAP）]] 的结构特征进行了表征。通过扫描电子显微镜确定了表面特征。通过紫外可见和荧光光谱确定了光学性质。结果发现，所得到的寡聚纳米粒子具有与单体不同的荧光特性，其量子产率为 15.90%，在 420nm 波长的光激发下发出绿色光，发射波长为 485nm。通过循环伏安法研究了寡聚纳米粒子的电化学能带隙，计算得到其电化学能带隙为 2.09eV。通过质量损失随温度升高的关系来确定寡聚材料的热稳定性。单体的热稳定性比聚合物高，无论是在最大质量损失发生的温度还是在加热后的残留量方面都是如此。
