Feng Jingyi, Gong Yue, Yang Shaoxiang, Tian Hongyu, Sun Baoguo
Beijing Key Laboratory of Flavor Chemistry, China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing, 100048, People's Republic of China.
Anal Sci. 2023 Nov;39(11):1909-1916. doi: 10.1007/s44211-023-00398-2. Epub 2023 Aug 7.
A colorimetric fluorescent probe (BTCNA) was developed for the determination of carboxylesterase and carbamate pesticides. The probe used naphthalene-benzothiazole as the fluorescent group and naphthyl acetate as the recognition group. The recognition mechanism of BTCNA for carboxylesterase was based on the enzymatic hydrolysis of naphthyl acetate by carboxylesterase (CES). The test paper of the BTCNA gradually changed from light blue to bright yellow with the increase of CES activity. The probe solution gradually changed from light blue to earth-yellow as the carbaryl concentration increased. There was a linear functional relationship between the RG (red, green) value of the photo and the CES activity. And a linear functional relationship between the carbaryl concentration and the RG value of the photo was found. Additionally, BTCNA was successfully used to detect the concentration of carbaryl in actual samples. BTCNA is a rapid detection tool for CES activity and carbamate pesticides using a smartphone.
开发了一种用于测定羧酸酯酶和氨基甲酸酯类农药的比色荧光探针（BTCNA）。该探针以萘并苯并噻唑作为荧光基团，乙酸萘酯作为识别基团。BTCNA对羧酸酯酶的识别机制基于羧酸酯酶（CES）对乙酸萘酯的酶促水解。随着CES活性的增加，BTCNA试纸从浅蓝色逐渐变为亮黄色。随着西维因浓度的增加，探针溶液从浅蓝色逐渐变为土黄色。照片的RG（红色、绿色）值与CES活性之间存在线性函数关系。并且发现西维因浓度与照片的RG值之间存在线性函数关系。此外，BTCNA已成功用于检测实际样品中西维因的浓度。BTCNA是一种使用智能手机检测CES活性和氨基甲酸酯类农药的快速检测工具。
