C-H Activation & Phytochemistry Lab, Chemical Technology Division CSIR-IHBT, Palampur, Himachal Pradesh 176061, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
C-H Activation & Phytochemistry Lab, Chemical Technology Division CSIR-IHBT, Palampur, Himachal Pradesh 176061, India.
Steroids. 2023 Nov;199:109293. doi: 10.1016/j.steroids.2023.109293. Epub 2023 Aug 6.
Four previously undescribed steroidal saponins named govanosides C-F (1-4) and nine known compounds (5-13) were isolated from the rhizomes of Trillium govanianum Wall. ex D.Don. Govanosides C-E contained a rare sugar moiety i.e., 6-deoxy allose, while govanoside F has acetylated rhamnose moiety in its glycan part. Also, this is the first report on the isolation of feruloyl sucrose derivatives (11-12) and (E)-4-hydroxy-dodec-2-enedioic acid (13) from the Trillium genus. The structure of isolated compounds was deduced using 1D and 2D NMR, HRESIMS, LC-MS/MS, GC-MS, and saccharide linkage analysis. Steroidal scaffold isolates (1-10) were evaluated for their antagonistic effects on acetylcholinesterase inhibitory activity. Govanoside C (1) significantly inhibited acetylcholinesterase (IC: 2.38 μM). Molecular docking experiments have also been performed to depict the molecule's interaction and binding free energy with acetylcholinesterase.
从独蒜兰的根茎中分离得到了四个以前未描述的甾体皂甙，分别命名为 govanoside C-F（1-4）和九个已知化合物（5-13）。Govanoside C-E 含有一个罕见的糖部分，即 6-去氧阿洛糖，而 govanoside F 在其糖苷部分则含有乙酰化鼠李糖部分。此外，这是首次从独蒜兰属植物中分离得到阿魏酸蔗糖衍生物（11-12）和（E）-4-羟基-十二-2-烯二酸（13）。通过 1D 和 2D NMR、HRESIMS、LC-MS/MS、GC-MS 和糖链分析推导出了分离化合物的结构。对分离得到的甾体骨架化合物（1-10）进行了乙酰胆碱酯酶抑制活性的拮抗作用评价。Govanoside C（1）对乙酰胆碱酯酶具有显著的抑制作用（IC：2.38 μM）。还进行了分子对接实验，以描绘分子与乙酰胆碱酯酶的相互作用和结合自由能。
