Klee G G, Shikegawa J, Trainer T D
Arch Pathol Lab Med. 1986 Jul;110(7):588-91.
Parathyroid hormone (PTH) analysis was recently added to the College of American Pathologists ligand assay survey. Synthetic whole-molecule PTH was used as the test hormone. This material was found to be stable in lyophilized serum for at least 10.7 days at 35 degrees C and 3.9 days at 45 degrees C. The survey results showed wide ranges of answers yielding within-assay coefficients of variation from 12% to 93%. The median percentage of the spiked hormone that was recovered by the various assay methods ranged from 24% to 390%. Separation of the spiked and endogenous measured PTH concentrations ranged from a twofold separation to an approximate 50% overlap of results. The survey may not be a valid indicator of PTH assay efficacy since whole-molecule human PTH may not be the most clinically important form of circulating PTH; however, the survey shows that there is a large heterogeneity in commercial PTH assays.
甲状旁腺激素（PTH）分析最近被纳入美国病理学家学会的配体分析调查。合成全分子PTH被用作检测激素。这种物质在冻干血清中于35℃下至少稳定10.7天，在45℃下稳定3.9天。调查结果显示答案范围广泛，批内变异系数在12%至93%之间。各种检测方法回收的加标激素的中位数百分比在24%至390%之间。加标和内源性测量的PTH浓度的分离范围从两倍分离到结果近似50%的重叠。该调查可能不是PTH检测效能的有效指标，因为全分子人PTH可能不是循环PTH最具临床重要性的形式；然而，该调查表明商业PTH检测存在很大的异质性。
