Wood W G
Dept. of Internal Medicine, Medical University of Lübeck, DE.
Scand J Clin Lab Invest Suppl. 1991;205:105-12.
This article deals with "matrix effects" in immunoassay systems. As each component has its own matrix (buffer, antibody, separation agents), the term matrix effect--usually only referring to the matrix of the sample--has been replaced by the term interference effect. The possible sites of interference have been dealt with in turn, taking each component/step of an immunoassay separately. Results of assay improvement due to the removal of "matrix effects" are given using the PTH-assay as example. The change in results due to tampering with the serum matrix is given using the determination of free thyroxine in commercial quality control sera.
本文探讨免疫分析系统中的“基质效应”。由于每个组分都有其自身的基质（缓冲液、抗体、分离剂），“基质效应”一词（通常仅指样品的基质）已被“干扰效应”一词所取代。已依次探讨了可能的干扰位点，分别针对免疫分析的每个组分/步骤进行分析。以甲状旁腺激素分析为例，给出了去除“基质效应”后分析改进的结果。以市售质量控制血清中游离甲状腺素的测定为例，给出了因血清基质被篡改而导致的结果变化。
