与预后不良相关的特定浸润性膀胱癌浸润 T 细胞亚群。
Specific subsets of urothelial bladder carcinoma infiltrating T cells associated with poor prognosis.
机构信息
College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing, 100029, China.
College of Life Science and Technology, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, Xinjiang, China.
出版信息
Sci Rep. 2023 Aug 7;13(1):12801. doi: 10.1038/s41598-023-39208-0.
Comprehensive investigation of tumor-infiltrating lymphocytes in cancer is crucial to explore the effective immunotherapies, but the composition of infiltrating T cells in urothelial bladder carcinoma (UBC) remains elusive. Here, single-cell RNA sequencing (scRNA-seq) were performed on total 30,905 T cells derived from peripheral blood, adjacent normal and tumor tissues from two UBC patients. We identified 18 distinct T cell subsets based on molecular profiles and functional properties. Specifically, exhausted T (T) cells, exhausted NKT (NKT) cells, Ki67 T cells and B cell-like T (B-T) cells were exclusively enriched in UBC. Additionally, the gene signatures of T, NKT, Ki67 T and B-T cells were significantly associated with poor survival in patients with BC and various tumor types. Finally, IKZF3 and TRGC2 are the potential biomarkers of T cells. Overall, our study demonstrated an exhausted context of T cells in UBC, which layed a theoretical foundation for the development of effective tumor immunotherapies.
全面研究肿瘤浸润淋巴细胞对于探索有效的免疫疗法至关重要，但膀胱癌（UBC）浸润 T 细胞的组成仍不清楚。在这里，对来自两名 UBC 患者的外周血、相邻正常组织和肿瘤组织的总共 30905 个 T 细胞进行了单细胞 RNA 测序（scRNA-seq）。我们根据分子特征和功能特性鉴定了 18 个不同的 T 细胞亚群。具体来说，耗竭的 T（T）细胞、耗竭的 NKT（NKT）细胞、Ki67 T 细胞和 B 细胞样 T（B-T）细胞仅在 UBC 中富集。此外，T、NKT、Ki67 T 和 B-T 细胞的基因特征与 BC 和各种肿瘤类型患者的不良生存显著相关。最后，IKZF3 和 TRGC2 是 T 细胞的潜在生物标志物。总的来说，我们的研究表明 UBC 中的 T 细胞处于耗竭状态，为开发有效的肿瘤免疫疗法奠定了理论基础。
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