Dietary Protein Research Group, Centre for Nutrition, Department of Clinical Medicine, University of Bergen, Haukeland University Hospital, 5021, Bergen, Norway.
Nofima, Bergen, Norway.
Eur J Nutr. 2023 Dec;62(8):3227-3240. doi: 10.1007/s00394-023-03227-4. Epub 2023 Aug 7.
The obese black and tan, brachyuric (BTBR) ob/ob mouse spontaneously develops features comparable to human diabetic nephropathy. The primary aim of the present study was to investigate if a diet containing fish proteins would attenuate or delay the development of glomerular hypertrophy (glomerulomegaly), mesangial sclerosis and albuminuria in obese BTBR ob/ob mice.
Obese BTBR.CgLep/WiscJ male mice were fed diets containing 25% of protein from Atlantic cod backbones and 75% of protein from casein (Cod-BB group), or casein as the sole protein source (control group). Kidneys were analysed morphologically, and markers for renal dysfunction were analysed biochemically in urine and serum.
The Cod-BB diet attenuated the development of mesangial sclerosis (P 0.040) without affecting the development of glomerular hypertrophy and albuminuria. The urine concentration of cystatin C (relative to creatinine) was lower in mice fed the Cod-BB diet (P 0.0044).
A diet containing cod backbone protein powder attenuated the development of mesangial sclerosis and tubular dysfunction in obese BTBR ob/ob mice, but did not prevent the development of glomerular hypertrophy and albuminuria in these mice.
肥胖的黑棕褐色、短肢（BTBR）ob/ob 小鼠自发发展出与人糖尿病肾病相当的特征。本研究的主要目的是研究是否含有鱼蛋白的饮食会减弱或延迟肥胖 BTBR.ob/ob 小鼠肾小球肥大（肾小球肿大）、系膜硬化和白蛋白尿的发展。
肥胖的 BTBR.CgLep/WiscJ 雄性小鼠喂食含有 25%大西洋鳕鱼骨架蛋白和 75%酪蛋白的饮食（Cod-BB 组），或仅用酪蛋白作为唯一蛋白来源（对照组）。对肾脏进行形态学分析，并在尿液和血清中分析肾功能障碍的标志物。
Cod-BB 饮食减弱了系膜硬化的发展（P 0.040），而不影响肾小球肥大和白蛋白尿的发展。Cod-BB 饮食组小鼠尿胱抑素 C（相对于肌酐）的浓度较低（P 0.0044）。
含有鳕鱼骨干蛋白粉的饮食减弱了肥胖 BTBR.ob/ob 小鼠系膜硬化和肾小管功能障碍的发展，但不能预防这些小鼠肾小球肥大和白蛋白尿的发展。
