• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

多组学揭示了小鼠卵丘细胞在卵母细胞成熟过程中的代谢模式。

Multi-omics reveal the metabolic patterns in mouse cumulus cells during oocyte maturation.

机构信息

State Key Laboratory of Reproductive Medicine, Suzhou Municipal Hospital, Nanjing Medical University, 101 Longmian Rd, Nanjing, Jiangsu, 211166, China.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

出版信息

J Ovarian Res. 2023 Aug 8;16(1):156. doi: 10.1186/s13048-023-01237-8.

DOI:10.1186/s13048-023-01237-8
PMID:37550748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10408154/
Abstract

Bi-directional communication between cumulus cells and the surrounded oocytes is important for the development and functions of both compartments. However, the metabolic framework in cumulus cells has not been systematically described. In the present study, cumulus cells from cumulus-oocyte complexes (COCs) at three key time points were isolated (arrested GV stage, post-hCG 0h; meiotic resumption GVBD stage, post-hCG 3h; and metaphase II stage, post-hCG 12h), and the temporal metabolomic and proteomic profiling were performed. Integrated multi-omics analysis reveals the global metabolic patterns in cumulus cells during mouse oocyte maturation. In particular, we found the active hyaluronic acid metabolism, steroid hormone synthesis, and prostaglandin E2 (PGE2) production in cumulus cells. Meanwhile, accompanying the oocyte maturation, a progressive increase in nucleotide and amino acid metabolism was detected in the surrounding cumulus cells. In sum, the data serve as a valuable resource for probing metabolism during terminal differentiation of ovarian granulosa cells, and provide the potential biomarkers for improving and predicting oocyte quality.

摘要

卵丘细胞与周围卵母细胞之间的双向通讯对两者的发育和功能都很重要。然而，卵丘细胞的代谢框架尚未得到系统描述。在本研究中，从卵丘-卵母细胞复合体（COCs）的三个关键时间点（GV 期阻滞，hCG 后 0 小时；减数分裂恢复 GVBD 期，hCG 后 3 小时；和 M 期，hCG 后 12 小时）分离出卵丘细胞，并进行了时间代谢组学和蛋白质组学分析。整合的多组学分析揭示了小鼠卵母细胞成熟过程中卵丘细胞的全局代谢模式。特别是，我们发现卵丘细胞中透明质酸代谢、类固醇激素合成和前列腺素 E2（PGE2）的产生非常活跃。同时，伴随着卵母细胞成熟，周围卵丘细胞中的核苷酸和氨基酸代谢逐渐增加。总之，这些数据为研究卵巢颗粒细胞终末分化过程中的代谢提供了有价值的资源，并为改善和预测卵母细胞质量提供了潜在的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/cf5c9951cb25/13048_2023_1237_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/8313a1ed669f/13048_2023_1237_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/2a5d0d93f876/13048_2023_1237_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/919f6d10e77d/13048_2023_1237_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/8674d13783c0/13048_2023_1237_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/1e970121a17b/13048_2023_1237_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/34f16ba6ac18/13048_2023_1237_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/91bb0e83b38c/13048_2023_1237_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/cf5c9951cb25/13048_2023_1237_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/8313a1ed669f/13048_2023_1237_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/2a5d0d93f876/13048_2023_1237_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/919f6d10e77d/13048_2023_1237_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/8674d13783c0/13048_2023_1237_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/1e970121a17b/13048_2023_1237_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/34f16ba6ac18/13048_2023_1237_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/91bb0e83b38c/13048_2023_1237_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56b/10408154/cf5c9951cb25/13048_2023_1237_Fig8_HTML.jpg

相似文献

1
Multi-omics reveal the metabolic patterns in mouse cumulus cells during oocyte maturation.多组学揭示了小鼠卵丘细胞在卵母细胞成熟过程中的代谢模式。
J Ovarian Res. 2023 Aug 8;16(1):156. doi: 10.1186/s13048-023-01237-8.
2
Endothelin-1 promotes human germinal vesicle-stage oocyte maturation by downregulating connexin-26 expression in cumulus cells.内皮素-1 通过下调卵丘细胞连接蛋白 26 的表达促进人卵母细胞生发泡期成熟。
Mol Hum Reprod. 2018 Jan 1;24(1):27-36. doi: 10.1093/molehr/gax058.
3
Temporal metabolomics analysis reveals the metabolic patterns in goat cumulus cells during oocyte maturation.时间代谢组学分析揭示了山羊卵丘细胞在卵母细胞成熟过程中的代谢模式。
Gene. 2024 Nov 30;928:148772. doi: 10.1016/j.gene.2024.148772. Epub 2024 Jul 16.
4
Characterization of metabolic patterns in porcine cumulus cells during meiotic maturation.猪卵丘细胞减数分裂成熟过程中代谢模式的特征。
Theriogenology. 2024 May;220:56-69. doi: 10.1016/j.theriogenology.2024.03.002. Epub 2024 Mar 7.
5
Cumulus cells of oocyte-cumulus complexes secrete a meiosis-activating substance when stimulated with FSH.卵母细胞-卵丘复合体的卵丘细胞在受到促卵泡激素刺激时会分泌一种减数分裂激活物质。
Mol Reprod Dev. 1997 Mar;46(3):296-305. doi: 10.1002/(SICI)1098-2795(199703)46:3<296::AID-MRD8>3.0.CO;2-K.
6
Proteolytic activity of the 26S proteasome is required for the meiotic resumption, germinal vesicle breakdown, and cumulus expansion of porcine cumulus-oocyte complexes matured in vitro.26S蛋白酶体的蛋白水解活性对于体外成熟的猪卵丘-卵母细胞复合体的减数分裂恢复、生发泡破裂和卵丘扩展是必需的。
Biol Reprod. 2008 Jan;78(1):115-26. doi: 10.1095/biolreprod.107.061366. Epub 2007 Oct 17.
7
Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production.卵丘细胞基因表达的差异表明了预成熟步骤对提高体外牛胚胎生产的益处。
Mol Hum Reprod. 2016 Dec;22(12):882-897. doi: 10.1093/molehr/gaw055. Epub 2016 Aug 24.
8
Comparative importance of fatty acid beta-oxidation to nuclear maturation, gene expression, and glucose metabolism in mouse, bovine, and porcine cumulus oocyte complexes.脂肪酸β氧化对小鼠、牛和猪卵丘-卵母细胞复合物核成熟、基因表达和葡萄糖代谢的相对重要性。
Biol Reprod. 2013 May 2;88(5):111. doi: 10.1095/biolreprod.113.108548. Print 2013 May.
9
A pre-in vitro maturation medium containing cumulus oocyte complex ligand-receptor signaling molecules maintains meiotic arrest, supports the cumulus oocyte complex and improves oocyte developmental competence.含有卵丘卵母细胞复合物配体-受体信号分子的预体外成熟培养基可维持减数分裂阻滞，支持卵丘卵母细胞复合物，并提高卵母细胞发育能力。
Mol Hum Reprod. 2017 Sep 1;23(9):594-606. doi: 10.1093/molehr/gax032.
10
Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep.颗粒细胞分泌的因子可提高绵羊小腔卵泡卵丘-卵母细胞复合体的发育能力。
PLoS One. 2020 Mar 17;15(3):e0229043. doi: 10.1371/journal.pone.0229043. eCollection 2020.

引用本文的文献

1
Integrated Multi-Omics Analysis Reveals Key Regulators of Bovine Oocyte Maturation.整合多组学分析揭示牛卵母细胞成熟的关键调控因子。
Int J Mol Sci. 2025 Apr 23;26(9):3973. doi: 10.3390/ijms26093973.
2
Multi-Omics Reveal the Metabolic Changes in Cumulus Cells During Aging.多组学揭示衰老过程中卵丘细胞的代谢变化。
Cell Prolif. 2025 Aug;58(8):e70014. doi: 10.1111/cpr.70014. Epub 2025 Mar 5.
3
Multi-omics approach to reveal follicular metabolic changes and their effects on oocyte competence in PCOS patients.多组学方法揭示多囊卵巢综合征患者卵泡代谢变化及其对卵母细胞能力的影响。

本文引用的文献

1
Proteomic Profiling Reveals the Molecular Control of Oocyte Maturation.蛋白质组学分析揭示了卵母细胞成熟的分子调控机制。
Mol Cell Proteomics. 2023 Jan;22(1):100481. doi: 10.1016/j.mcpro.2022.100481. Epub 2022 Dec 7.
2
Biofilm Inhibiting Activity of Advanced Glycation Endproduct Crosslink Breaking and Glycation Inhibiting Compounds.晚期糖基化终产物交联断裂及糖基化抑制化合物的生物膜抑制活性
Antibiotics (Basel). 2022 Oct 14;11(10):1412. doi: 10.3390/antibiotics11101412.
3
A Comparative Study of Hesperetin, Hesperidin and Hesperidin Glucoside: Antioxidant, Anti-Inflammatory, and Antibacterial Activities In Vitro.
Front Endocrinol (Lausanne). 2024 Oct 11;15:1426517. doi: 10.3389/fendo.2024.1426517. eCollection 2024.
橙皮素、橙皮苷和橙皮苷葡萄糖苷的比较研究：体外抗氧化、抗炎和抗菌活性
Antioxidants (Basel). 2022 Aug 20;11(8):1618. doi: 10.3390/antiox11081618.
4
Dysregulated sphingolipid metabolism and autophagy in granulosa cells of women with endometriosis.子宫内膜异位症患者颗粒细胞中鞘脂代谢和自噬的失调。
Front Endocrinol (Lausanne). 2022 Aug 3;13:906570. doi: 10.3389/fendo.2022.906570. eCollection 2022.
5
Biological Synthesis of Bioactive Gold Nanoparticles from for Dual Chemo-Photothermal Effects against Human Brain Cancer Cells.从 中生物合成具有双重化疗-光热效应的生物活性金纳米粒子用于人类脑癌细胞。
Int J Mol Sci. 2022 Feb 18;23(4):2292. doi: 10.3390/ijms23042292.
6
Utilization of Rosmarinic and Ascorbic Acids for Maturation Culture Media in Order to Increase Sow Oocyte Quality Prior to IVF.利用迷迭香酸和抗坏血酸作为成熟培养液，提高 IVF 前母猪卵母细胞质量。
Molecules. 2021 Nov 28;26(23):7215. doi: 10.3390/molecules26237215.
7
Generation of offspring-producing 3D ovarian organoids derived from female germline stem cells and their application in toxicological detection.由雌性生殖干细胞生成的具有生育能力的 3D 卵巢类器官及其在毒理学检测中的应用。
Biomaterials. 2021 Dec;279:121213. doi: 10.1016/j.biomaterials.2021.121213. Epub 2021 Oct 21.
8
Progesterone affects clinic oocyte yields by coordinating with follicle stimulating hormone via PI3K/AKT and MAPK pathways.孕激素通过与卵泡刺激素通过 PI3K/AKT 和 MAPK 通路的相互作用来影响临床卵子的产量。
J Adv Res. 2021 Mar 2;33:189-199. doi: 10.1016/j.jare.2021.02.008. eCollection 2021 Nov.
9
clusterProfiler 4.0: A universal enrichment tool for interpreting omics data.clusterProfiler 4.0：用于解释组学数据的通用富集工具。
Innovation (Camb). 2021 Jul 1;2(3):100141. doi: 10.1016/j.xinn.2021.100141. eCollection 2021 Aug 28.
10
Reprogramming of glucose metabolism of cumulus cells and oocytes and its therapeutic significance.卵丘细胞和卵母细胞葡萄糖代谢的重编程及其治疗意义。
Reprod Sci. 2022 Mar;29(3):653-667. doi: 10.1007/s43032-021-00505-6. Epub 2021 Mar 5.