Kassim Yassin, Sheng Hao, Xu Guangjun, Jin Hao, Iqbal Tariq, Elashry Mostafa, Zhang Kun
Key Laboratory of Dairy Cow Genetic Improvement and Milk Quality Research of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Department of Animal and Poultry Production, Faculty of Agriculture, Minia University, El-Minya 61519, Egypt.
Int J Mol Sci. 2025 Apr 23;26(9):3973. doi: 10.3390/ijms26093973.
A well-regulated metabolism is crucial for optimal oocyte development and embryonic health. However, the metabolic framework governing oocyte maturation remains poorly understood. Using bovine oocytes as a model, we examined metabolomic and transcriptomic alterations during the transition from the germinal vesicle (GV) to the metaphase II (MII) stage. Our findings reveal distinct metabolic shifts, including suppressed β-oxidation combined with the accumulation of long-chain fatty acids (LCFAs). Notably, progesterone emerged as a key regulator of meiotic resumption through its influence on cAMP levels. We also observed enhanced glycolysis, moderate activation of the citric acid cycle (TCA cycle), and suppression of oxidative phosphorylation (OXPHOS), alongside reduced urea cycle flux and shifts in amino acid metabolism favoring glutamate synthesis. Intriguingly, discrepancies between metabolic and transcriptional activities in pathways such as the TCA cycle and nucleotide metabolism suggest asynchronous regulation. These findings provide a comprehensive multi-omics resource, advancing our understanding of the dynamic metabolic and transcriptional landscape during bovine oocyte maturation.
良好调节的新陈代谢对于最佳的卵母细胞发育和胚胎健康至关重要。然而，关于卵母细胞成熟的代谢框架仍知之甚少。我们以牛卵母细胞为模型，研究了从生发泡（GV）期到中期II（MII）期转变过程中的代谢组学和转录组学变化。我们的研究结果揭示了明显的代谢转变，包括β-氧化受到抑制以及长链脂肪酸（LCFA）的积累。值得注意的是，孕酮通过影响cAMP水平，成为减数分裂恢复的关键调节因子。我们还观察到糖酵解增强、柠檬酸循环（TCA循环）适度激活、氧化磷酸化（OXPHOS）受到抑制，同时尿素循环通量降低以及氨基酸代谢向有利于谷氨酸合成的方向转变。有趣的是，TCA循环和核苷酸代谢等途径中的代谢活动与转录活动之间的差异表明存在异步调节。这些发现提供了全面的多组学资源，增进了我们对牛卵母细胞成熟过程中动态代谢和转录格局的理解。
