Nemeth E F, Scarpa A
FEBS Lett. 1986 Jul 14;203(1):15-9. doi: 10.1016/0014-5793(86)81427-2.
The concentration of intracellular Ca2+ [( Ca2+]i) was measured in dissociated bovine parathyroid cells loaded with quin-2 or fura-2. In quin-2-loaded cells, increases in the concentration of extracellular Ca2+ elicited slow, monophasic increases in [Ca2+]i, whereas in fura-2-loaded cells, extracellular Ca2+ evoked rapid, transient increases which were followed by lower, yet sustained increases in [Ca2+]i. Cytosolic Ca2+ transients arose from the mobilization of cellular Ca2+ and could be evoked by a variety of divalent cations. Transient, but not sustained increases in [Ca2+]i were associated with an inhibition of hormone secretion. Secretion was still inhibited, however, when cytosolic Ca2+ transients were blocked by buffering with quin-2, suggesting that changes in [Ca2+]i might not be the essential factor regulating secretion in parathyroid cells.
在装载有喹啉-2或氟罗-2的解离牛甲状旁腺细胞中测量细胞内Ca2+浓度([Ca2+]i)。在装载喹啉-2的细胞中，细胞外Ca2+浓度的增加引起[Ca2+]i缓慢的单相增加，而在装载氟罗-2的细胞中，细胞外Ca2+引起快速的瞬时增加，随后是[Ca2+]i较低但持续的增加。胞质Ca2+瞬变源于细胞内Ca2+的动员，并且可以由多种二价阳离子诱发。[Ca2+]i的瞬时而非持续增加与激素分泌的抑制有关。然而，当用喹啉-2缓冲来阻断胞质Ca2+瞬变时，分泌仍然受到抑制，这表明[Ca2+]i的变化可能不是调节甲状旁腺细胞分泌的关键因素。
