Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Food Funct. 2023 Sep 19;14(18):8201-8216. doi: 10.1039/d3fo00613a.
Activation of adipose tissue thermogenesis is a promising strategy in the treatment of obesity and obesity-related metabolic disorders. Kaempferol (KPF) is a predominant dietary flavonoid with multiple pharmacological properties, such as anti-inflammatory and antioxidant activities. In this study, we sought to characterize the role of KPF in adipocyte thermogenesis. We demonstrated that KPF-treated mice were protected from diet-induced obesity, glucose tolerance, and insulin resistance, accompanied by markedly increased energy expenditure, oxygen consumption of white fat, and increased expression of proteins related to adaptive thermogenesis. KPF-promoted beige cell formation is a cell-autonomous effect, since the overexpression of cyclin-dependent kinase 6 (CDK6) in preadipocytes partially reversed browning phenotypes observed in KPF-treated cells. Overall, these data implicate that KPF is involved in promoting beige cell formation by suppressing CDK6 protein expression. This study provides evidence that KPF is a promising natural product for obesity treatment by boosting energy expenditure.
脂肪组织产热的激活是治疗肥胖和肥胖相关代谢紊乱的一种很有前途的策略。山奈酚（KPF）是一种主要的膳食类黄酮，具有多种药理作用，如抗炎和抗氧化活性。在这项研究中，我们试图描述 KPF 在脂肪细胞产热中的作用。我们证明，KPF 处理的小鼠可以预防饮食诱导的肥胖、葡萄糖耐量和胰岛素抵抗，同时伴随着能量消耗、白色脂肪的耗氧量显著增加，以及与适应性产热相关的蛋白质表达增加。KPF 促进米色细胞形成是一种细胞自主效应，因为在脂肪前体细胞中过表达细胞周期蛋白依赖性激酶 6（CDK6）部分逆转了 KPF 处理细胞中观察到的褐色表型。总的来说，这些数据表明 KPF 通过抑制 CDK6 蛋白表达参与促进米色细胞形成。这项研究提供的证据表明，KPF 通过增加能量消耗，是一种有前途的肥胖治疗天然产物。
