School of Biology and Ecology, University of Maine, Orono, ME 04469.
Plant Dis. 2024 Jan;108(1):182-189. doi: 10.1094/PDIS-07-22-1684-RE. Epub 2024 Jan 24.
The fungus (Mvc) causes mummy berry disease in blueberries including lowbush blueberry, , and is a significant pathogen of concern for Maine lowbush blueberry growers. This disease is typically managed with fungicides or by burning of plant debris containing overwintering pseudosclerotia. The population structure of Mvc in various fields in Maine was investigated using microsatellites and isolates collected from three stages in the Mvc lifecycle. The impacts of management strategies were also examined. A high level of genetic diversity was observed in Mvc from 12 lowbush blueberry fields with 199 unique multilocus haplotypes (MLHs) occurring in an original sample of 232 isolates. Twelve private alleles, including six private alleles with frequencies above 0.05, which indicated gene flow, were observed in six out of 12 fields. The population of Mvc in Maine as a whole is mostly a sexual, outcrossing population, as was seen in the diversity of MLHs and low amounts of linkage disequilibrium, although some apothecia appear to result from selfing. Three fields appear to have some clonal reproduction but were not strictly clonal, as multiple MLHs were noted in these fields. Management does not appear to affect population structure, and Mvc may be one large statewide population in Maine.
这种真菌（Mvc）会引起包括矮蓝莓在内的蓝莓果实真菌病，是缅因州矮蓝莓种植者关注的重要病原菌。这种疾病通常通过杀菌剂或焚烧含有越冬假菌核的植物残体来管理。使用微卫星和从 Mvc 生命周期的三个阶段收集的分离物，调查了缅因州不同田地中 Mvc 的种群结构。还检查了管理策略的影响。在来自 12 个矮蓝莓田的 232 个分离物的原始样本中，观察到 199 个独特的多位点单倍型（MLH），表明 Mvc 具有高水平的遗传多样性。在 12 个田地中的 6 个中观察到 12 个特有等位基因，包括 6 个频率高于 0.05 的特有等位基因，表明存在基因流。缅因州 Mvc 的整体种群主要是有性的异交种群，这从 MLH 的多样性和低程度的连锁不平衡中可以看出，尽管一些子囊果似乎是自交的结果。三个田地似乎有一些克隆繁殖，但不是严格的克隆繁殖，因为在这些田地中注意到了多个 MLH。管理似乎不会影响种群结构，而 Mvc 可能是缅因州全州范围内的一个大种群。
