Multiple Applications of a Novel Fluorescence Probe with Large Stokes Shift and Sensitivity for Rapid HS Detection.

Herein, a novel fluorescence probe Fla-DNP based on flavonol has been designed and synthesized for rapid, specific detection of HS. With the addition of HS, Fla-DNP triggered thiolysis and released Fla displaying the "turn-on" fluorescence response at 566 nm, which is consistent with the reaction site predicted by calculating Electrostatic potential and ADCH charges. As an easily available HS probe, Fla-DNP has the advantages of high selectivity, anti-interference, low detection limit (0.834 μM), short response time (6 min), and large Stokes shift (124 nm). The sensing mechanism of HS was determined by HRMS analysis and DFT calculation. Moreover, Fla-DNP processes a wide range of multiple applications, including the detection of HS in environmental water samples with good recovery rates ranging from 89.6% to 102.0%, as well as tracking the production of HS during food spoilage. Meanwhile, the probe exhibits superior biocompatibility and can not only be available used for HS detection in living cells but be further designed as an HS-activated CO photoreleaser, based on which it can be developed as a targeted anti-cancer drug.