Yu Lina, Tian Zhe, Joshi Dev Raj, Yuan Lin, Tuladhar Reshma, Zhang Yu, Yang Min
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100190, China.
ACS ES T Water. 2022 May 10;2(11):2175-2184. doi: 10.1021/acsestwater.2c00079. eCollection 2022 Nov 11.
This study aimed to provide a low-cost technique for virus detection in wastewater by improving an aluminum hydroxide adsorption-precipitation method. The releasing efficiency of viruses trapped by the aluminum hydroxide precipitates was improved by adding ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) to dissolve the precipitates at a NaEDTA·2HO:AlCl molar ratio of 1.8-3.6. The recovery rates of the improved method for seven viruses, including SARS-CoV-2-abEN pseudovirus and six animal viruses, were 5.9-22.3% in tap water and 4.9-35.1% in wastewater. Rotavirus A (9.0-4.5 × 10 copies/mL), porcine circovirus type 2 (5.8-6.4 × 10 copies/mL), and porcine parvovirus (5.6-2.7 × 10 copies/mL) were detected in China's pig farm wastewater, while rotavirus A (2.0 × 10 copies/mL) was detected in hospital wastewater. SARS-CoV-2 was detected in hospital wastewater (8.4 × 10 to 1.4 × 10 copies/mL), sewage (6.4 × 10 to 2.3 × 10 copies/mL), and river water (6.6 × 10 to 9.3 × 10 copies/mL) in Nepal. The method was automized, with a rate of recovery of 4.8 ± 1.4% at a virus concentration of 10 copies/mL. Thus, the established method could be used for wastewater-based epidemiology with sufficient sensitivity in coping with the COVID-19 epidemic and other virus epidemics.
本研究旨在通过改进氢氧化铝吸附沉淀法，提供一种用于检测废水中病毒的低成本技术。通过添加乙二胺四乙酸二钠盐（EDTA-2Na）以NaEDTA·2H₂O:AlCl摩尔比为1.8 - 3.6溶解沉淀物，提高了被氢氧化铝沉淀物捕获的病毒的释放效率。改进方法对包括SARS-CoV-2-abEN假病毒和六种动物病毒在内的七种病毒的回收率，在自来水中为5.9 - 22.3%，在废水中为4.9 - 35.1%。在中国的猪场废水中检测到了A组轮状病毒（9.0 - 4.5×10⁴拷贝/mL）、猪圆环病毒2型（5.8 - 6.4×10⁴拷贝/mL）和猪细小病毒（5.6 - 2.7×10⁴拷贝/mL），而在医院废水中检测到了A组轮状病毒（2.0×10⁴拷贝/mL）。在尼泊尔的医院废水（8.4×10³至1.4×10⁴拷贝/mL）、污水（6.4×10³至2.3×10⁴拷贝/mL）和河水中检测到了SARS-CoV-2。该方法实现了自动化，在病毒浓度为10³拷贝/mL时回收率为4.8±1.4%。因此，所建立的方法可用于基于废水的流行病学研究，在应对新冠疫情和其他病毒疫情方面具有足够的敏感性。
