Denhardt D T, Hamilton R T, Parfett C L, Edwards D R, St Pierre R, Waterhouse P, Nilsen-Hamilton M
Cancer Res. 1986 Sep;46(9):4590-3.
Complementary DNA clones corresponding to 638 nucleotides of the messenger RNA encoding the major portion of murine major excreted protein have been isolated and sequenced. The amino acid sequence of a part of the murine major excreted protein deduced from the DNA sequence reveals substantial and significant homology with the cysteine proteases actinidin, rat cathepsin H, and papain. Since the amount of murine major excreted protein secreted by cultured cells is often enhanced by transformation, it is implicated in oncogenic phenomena and may play a role in the metastatic process by virtue of its proteolytic activity.
已分离并测序了与编码小鼠主要排泄蛋白大部分的信使核糖核酸638个核苷酸相对应的互补DNA克隆。从DNA序列推导出来的小鼠主要排泄蛋白部分的氨基酸序列显示，它与半胱氨酸蛋白酶肌动蛋白、大鼠组织蛋白酶H和木瓜蛋白酶有大量且显著的同源性。由于培养细胞分泌的小鼠主要排泄蛋白的量常常因转化而增加，所以它与致癌现象有关，并且可能因其蛋白水解活性在转移过程中发挥作用。
