Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, College of Life Science, Nanchang University, Jiangxi 330031, China.
Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
Mol Plant. 2023 Aug 7;16(8):1339-1353. doi: 10.1016/j.molp.2023.08.001.
Tens of thousands of long non-coding RNAs have been uncovered in plants, but few of them have been comprehensively studied for their biological function and molecular mechanism of their mode of action. Here, we show that the Arabidopsis long non-coding RNA DANA2 interacts with an AP2/ERF transcription factor ERF84 in the cell nucleus and then affects the transcription of JMJ29 that encodes a Jumonji C domain-containing histone H3K9 demethylase. Both RNA sequencing (RNA-seq) and genetic analyses demonstrate that DANA2 positively regulates drought stress responses through JMJ29. JMJ29 positively regulates the expression of ERF15 and GOLS2 by modulation of H3K9me2 demethylation. Accordingly, mutation of JMJ29 causes decreased ERF15 and GOLS2 expression, resulting in impaired drought tolerance, in agreement with drought-sensitive phenotypes of dana2 and erf84 mutants. Taken together, these results demonstrate that DANA2 is a positive regulator of drought response and works jointly with the transcriptional activator ERF84 to modulate JMJ29 expression in plant response to drought.
在植物中已经发现了数以万计的长非编码 RNA，但对它们的生物学功能及其作用模式的分子机制进行全面研究的却很少。在这里，我们表明拟南芥长非编码 RNA DANA2 在细胞核内与 AP2/ERF 转录因子 ERF84 相互作用，然后影响编码具有 Jumonji C 结构域的组蛋白 H3K9 去甲基化酶 JMJ29 的转录。RNA 测序（RNA-seq）和遗传分析表明，DANA2 通过 JMJ29 正向调控干旱胁迫反应。JMJ29 通过调节 H3K9me2 去甲基化正向调控 ERF15 和 GOLS2 的表达。因此，JMJ29 的突变导致 ERF15 和 GOLS2 的表达减少，从而导致耐旱性降低，与 dana2 和 erf84 突变体的干旱敏感表型一致。总之，这些结果表明 DANA2 是干旱反应的正调节剂，与转录激活因子 ERF84 一起在植物对干旱的反应中共同调节 JMJ29 的表达。
