Mansouri Esrafil, Shafiei Seifabadi Zeinab, Azarbarz Nastaran, Zare Moaiedi Maasoumeh
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Drug Chem Toxicol. 2024 Mar;47(2):227-234. doi: 10.1080/01480545.2023.2242008. Epub 2023 Aug 8.
In recent years, the cardiotoxicity and hepatotoxicity induced by chemotherapeutic drugs such as cisplatin (CP) have become significant issues. The current research looks into the effects of sodium hydrosulfide (NaHS) on CP-induced hepatotoxicity and cardiotoxicity in rats. A total of 32 male Sprague Dawley rats were separated into four different groups: (1) control group, received only normal saline; (2) NaHS group, was intraperitoneally injected with NaHS (200 µg/kg/d, dissolved in saline) for 15 days; (3) CP group, was intraperitoneally injected only one dose of CP (5 mg/kg) and (4) CP plus NaHS group, received CP along with NaHS. Blood and tissues samples were harvested for biochemical, histopathological, and immunohistochemical investigations. To determine the data's statistical significance, a one-way analysis of variance was used. CP injection significantly increased alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), Creatine phospho kinase (CK-MB), cholesterol, low-density lipoprotein (LDL), triglyceride (TG), and lipid peroxidation levels, while high-density lipoprotein (HDL), albumin, glutathione peroxidase, superoxide dismutase, and catalase (CAT) levels were significantly reduced with pathological alterations in liver and heart tissues. Co-treatment NaHS with CP ameliorates the biochemical and histological parameters. Also, Treatment solely with CP resulted in increased tissue expression of (IL-1β) in liver and heart but co-treatment NaHS with CP reduced the expression of this inflammatory factor. We conclude that NaHS operates in the liver and heart as an anti-inflammatory and powerful free radicals' scavenger to inhibit the toxic effects of CP, both at the biochemical and histopathological levels.
近年来，顺铂（CP）等化疗药物引起的心脏毒性和肝脏毒性已成为重大问题。当前研究探讨了硫化氢钠（NaHS）对CP诱导的大鼠肝脏毒性和心脏毒性的影响。总共32只雄性Sprague Dawley大鼠被分为四组：（1）对照组，仅接受生理盐水；（2）NaHS组，腹腔注射NaHS（200 µg/kg/d，溶于生理盐水），持续15天；（3）CP组，仅腹腔注射一剂CP（5 mg/kg）；（4）CP + NaHS组，接受CP和NaHS。采集血液和组织样本进行生化、组织病理学和免疫组织化学研究。为确定数据的统计学意义，采用单因素方差分析。注射CP显著提高了丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、肌酸磷酸激酶（CK-MB）、胆固醇、低密度脂蛋白（LDL）、甘油三酯（TG）和脂质过氧化水平，而高密度脂蛋白（HDL）、白蛋白、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶（CAT）水平显著降低，同时肝脏和心脏组织出现病理改变。NaHS与CP联合治疗改善了生化和组织学参数。此外，单独使用CP治疗导致肝脏和心脏中白细胞介素-1β（IL-1β）的组织表达增加，但NaHS与CP联合治疗降低了这种炎症因子的表达。我们得出结论，NaHS在肝脏和心脏中作为一种抗炎和强大的自由基清除剂发挥作用，在生化和组织病理学水平上抑制CP的毒性作用。
