Department of Anatomical Sciences, Abadan University of Medical Sciences, Abadan, Iran.
Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Trace Elem Med Biol. 2023 Dec;80:127306. doi: 10.1016/j.jtemb.2023.127306. Epub 2023 Sep 22.
Alpinia officinarum is a member of the ginger family (Zingiberaceae), which is widely cultivated in Asia and traditionally used for its anti-inflammatory, antimicrobial, and antihyperlipidemic qualities. This study aimed to evaluate the effect of Alpinia officinarum rhizome extract (AORE) on cisplatin (CP)-induced hepatotoxicity in rats.
Forty-four male rats were divided into six groups including the control group, AORE control group, CP control group, and three groups of CP (7 mg/kg dose, on the 10th day) with AORE (at concentrations of 100, 200 and 400 mg/kg, daily for 14 days). After 14 days, the rats' livers were removed and their liver function was assessed using biochemical marker enzymes including serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) activities and albumin, total protein, and total bilirubin (T. bilirubin). Oxidative stress was assessed by evaluating malondialdehyde concentration and hepatic superoxide dismutase activity, histopathological and immunohistochemical tests were also conducted.
Results demonstrated that treatment with AORE reduced the toxicity in levels of the hepatic biomarkers in cp-induced groups. AORE treatment decreased oxidative stress and improved histopathological indexes. Furthermore, immunohistochemical (IHC) investigation showed the B-cell lymphoma 2 (Bcl-2) upsurging and p53 downregulating expression exhibiting the recovery following AORE administration.
The founding suggested that AORE administration has positive biochemical, histopathological, and immunohistochemical impacts on the ameliorating of hepatotoxicity in CP-induced rats.
益智是姜科（Zingiberaceae）的一员，广泛种植于亚洲，传统上用于抗炎、抗菌和抗高血脂。本研究旨在评估益智根茎提取物（AORE）对顺铂（CP）诱导的大鼠肝毒性的影响。
44 只雄性大鼠分为 6 组，包括对照组、AORE 对照组、CP 对照组和 3 组 CP（7mg/kg 剂量，第 10 天）加 AORE（浓度分别为 100、200 和 400mg/kg，每天 14 天）。14 天后，取出大鼠肝脏，用生化标志物酶评估其肝功能，包括血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）活性以及白蛋白、总蛋白和总胆红素（T.胆红素）。通过评估丙二醛浓度和肝超氧化物歧化酶活性来评估氧化应激，还进行了组织病理学和免疫组织化学测试。
结果表明，AORE 治疗降低了 CP 诱导组肝生物标志物的毒性。AORE 治疗降低了氧化应激，改善了组织病理学指标。此外，免疫组织化学（IHC）研究表明，B 细胞淋巴瘤 2（Bcl-2）上调和 p53 下调表达在 AORE 给药后得到恢复。
研究结果表明，AORE 给药对 CP 诱导的大鼠肝毒性具有积极的生化、组织病理学和免疫组织化学影响。
