Department of Anatomy, National University of Medical Sciences, Rawalpindi, Pakistan.
Department of Histopathology, National University of Medical Sciences, Rawalpindi, Pakistan.
J Coll Physicians Surg Pak. 2023 Aug;33(8):836-841. doi: 10.29271/jcpsp.2023.08.836.
To determine the ameliorative effects of prolotherapy on monosodium iodoacetate (MIA) induced and histomorphological changes in the articular cartilage of tibial condyles at rat knee joint.
An experimental study. Place and Duration of the Study: Department of Anatomy, Army Medical College Rawalpindi, NUMS, Rawalpindi, from August to November 2021.
Thirty adult male Sprague Dawley rats were divided into three groups, each having 10 rats. Group A was control. Group B was injected with single dose of 1mg MIA intraarticularly in the right knee to induce osteoarthritic changes. Group C was injected with single dose of 1mg MIA intraarticularly, in right knee was followed by 0.1ml Prolotherapy (3ml of 25% dextrose, 2ml of 2% xylocaine, 1ml of injection neurobion, and 1ml of injection methecobal) as intra articular injection at week 2, 6 and 10 in right knee. Rats were sacrificed after one month of the last dose of Prolotherapy. Articular cartilage was collected for gross and histological examination and compared among the groups.
Articular cartilage belonging to control group A was normal. While group B showed statistically significant deterioration in gross appearance (p = 0.001**), reduction in number of chondrocytes (p = 0.005*) and thickness of articular cartilage (p = 0.001**) in comparison to group A. In group C due to prolotherapy statistically significant improvement in gross appearance (p = 0.034*), increase in number of chondrocytes (p = 0.003*), and thickness of articular cartilage (p = 0.001**) was observed as compared to group B.
Prolotherapy significantly ameliorates histomorphology of tibial articular cartilage against MIA induced osteoarthritic changes in rat knee joint.
Articular cartilage, Knee joint, Monosodium iodoacetate, Osteoarthritis, Prolotherapy.
确定增生疗法对单碘乙酸（MIA）诱导的大鼠膝关节胫骨髁关节软骨的改善作用和组织形态学变化。
实验研究。地点和研究时间：陆军医科大学解剖学系，NUMS，拉瓦尔品第，2021 年 8 月至 11 月。
将 30 只成年雄性 Sprague Dawley 大鼠分为三组，每组 10 只。A 组为对照组。B 组在右膝关节内单次注射 1mg MIA，诱导骨关节炎变化。C 组在右膝关节内单次注射 1mg MIA，然后在第 2、6 和 10 周在右膝关节内注射 0.1ml 增生疗法（3ml 25%葡萄糖、2ml 2%利多卡因、1ml 注射神经生物素和 1ml 注射甲钴胺）。最后一次注射增生疗法一个月后处死大鼠。收集关节软骨进行大体和组织学检查，并比较各组之间的差异。
A 组（对照组）的关节软骨正常。而 B 组在大体外观（p = 0.001**）、软骨细胞数量（p = 0.005*）和关节软骨厚度（p = 0.001**）方面与 A 组相比有统计学显著恶化。在 C 组（增生疗法组）中，由于增生疗法，在大体外观（p = 0.034*）、软骨细胞数量（p = 0.003*）和关节软骨厚度（p = 0.001**）方面有统计学显著改善。
增生疗法可显著改善 MIA 诱导的大鼠膝关节骨关节炎关节软骨的组织形态学。
关节软骨；膝关节；单碘乙酸；骨关节炎；增生疗法。
