Hanko J H, Törnebrandt K, Hardebo J E, Kåhrström J, Nobin A, Owman C
J Auton Pharmacol. 1986 Jun;6(2):117-24. doi: 10.1111/j.1474-8673.1986.tb00638.x.
Neuropeptide Y (NPY) has recently been reported to coexist with noradrenaline (NA) in central as well as peripheral noradrenergic nerves. NPY-containing nerve fibres are particularly numerous around blood vessels. Studies were performed on isolated pial arteries as well as on arteries and veins from several peripheral vascular beds from rabbit, cat and man. NPY induced a varying degree of direct contraction of the vessels with an EAm up to 15 mN. Pial arteries were more sensitive than peripheral arteries to NPY (mean EC50 = 7.6 X 10(-9) M). The presence of NPY did not cause any consistent or significant potentiation of the contractile response to NA in any of the vessels tested. Transmural electrical stimulation of the perivascular nerves (including blockade with tetrodotoxin) was performed mainly with auricular artery from the rabbit. Blocking experiments confirmed that the neurogenic contraction was mediated by noradrenergic-type fibres. NPY caused a concentration-related potentiation of the neurally evoked contractile response. The peptide also potentiated the tetrodotoxin-resistant probably non-neurogenic contractions obtained during enhanced electrical field stimulation. It is concluded that NPY interacts with NA during sympathetic nerve activation primarily through a presynaptic effect.
最近有报道称，神经肽Y（NPY）与去甲肾上腺素（NA）共存于中枢和外周的去甲肾上腺素能神经中。含NPY的神经纤维在血管周围尤其丰富。我们对兔、猫和人的软脑膜动脉以及来自几个外周血管床的动脉和静脉进行了研究。NPY可使血管产生不同程度的直接收缩，最大收缩张力可达15 mN。软脑膜动脉对NPY比外周动脉更敏感（平均EC50 = 7.6×10⁻⁹ M）。在所测试的任何血管中，NPY的存在均未对NA的收缩反应产生任何持续或显著的增强作用。主要使用兔的耳动脉进行血管周围神经的跨壁电刺激（包括用河豚毒素阻断）。阻断实验证实神经源性收缩是由去甲肾上腺素能型纤维介导的。NPY可使神经诱发的收缩反应产生浓度依赖性增强。该肽还增强了在增强电场刺激期间获得的对河豚毒素耐药的可能非神经源性收缩。结论是，在交感神经激活过程中，NPY主要通过突触前效应与NA相互作用。
