Tickell Kirkby D, Denno Donna M, Saleem Ali, Kazi Zaubina, Singa Benson O, Achieng Catherine, Mutinda Charles, Richardson Barbra A, Ásbjörnsdóttir Kristjana H, Hawes Stephen E, Berkley James A, Walson Judd L
Department of Global Health, University of Washington, Seattle, WA, USA.
The Childhood Acute Illness & Nutrition (CHAIN) Network, Nairobi, Kenya.
iScience. 2023 Jul 11;26(8):107294. doi: 10.1016/j.isci.2023.107294. eCollection 2023 Aug 18.
We aimed to establish if enteric permeability was associated with similar biological processes in children recovering from hospitalization and relatively healthy children in the community. Extreme gradient boosted models predicting the lactulose-rhamnose ratio (LRR), a biomarker of enteric permeability, using 7,500 plasma proteins and 34 fecal biomarkers of enteric infection among 89 hospitalized and 60 community children aged 2-23 months were built. The R values were calculated in test sets. The models performed better among community children (R: 0.27 [min-max: 0.19, 0.53]) than hospitalized children (R: 0.07 [min-max: 0.03, 0.11]). In the community, LRR was associated with biomarkers of humoral antimicrobial and cellular lipopolysaccharide responses and inversely associated with anti-inflammatory and innate immunological responses. Among hospitalized children, the selected biomarkers had few shared functions. This suggests enteric permeability among community children was associated with a host response to pathogens, but this association was not observed among hospitalized children.
我们旨在确定肠道通透性是否与住院康复儿童和社区中相对健康儿童的相似生物学过程相关。构建了极端梯度提升模型，该模型使用89名住院儿童和60名2至23个月大的社区儿童中的7500种血浆蛋白和34种肠道感染粪便生物标志物来预测乳果糖-鼠李糖比率（LRR），这是一种肠道通透性的生物标志物。在测试集中计算R值。这些模型在社区儿童中表现更好（R：0.27 [最小值-最大值：0.19，0.53]），而在住院儿童中表现较差（R：0.07 [最小值-最大值：0.03，0.11]）。在社区中，LRR与体液抗菌和细胞脂多糖反应的生物标志物相关，与抗炎和先天免疫反应呈负相关。在住院儿童中，所选生物标志物几乎没有共同功能。这表明社区儿童的肠道通透性与宿主对病原体的反应相关，但在住院儿童中未观察到这种关联。
