全基因组分析揭示了中国广西[具体研究对象]的高遗传多样性和耐药特征。 （注：原文中“in Guangxi, China”前面缺少具体的研究对象，翻译时根据语境补充了[具体研究对象]）
Whole Genomic Analysis Revealed High Genetic Diversity and Drug-Resistant Characteristics of in Guangxi, China.
作者信息
Liang Dabin, Song Zexuan, Liang Xiaoyan, Qin Huifang, Huang Liwen, Ye Jing, Lan Rushu, Luo Dan, Zhao Yanlin, Lin Mei
机构信息
Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning, Guangxi, People's Republic of China.
Guangxi Key Laboratory of Major Infectious Disease Prevention and Control and Biosafety Emergency Response, Nanning, Guangxi, People's Republic of China.
出版信息
Infect Drug Resist. 2023 Aug 3;16:5021-5031. doi: 10.2147/IDR.S410828. eCollection 2023.
BACKGROUND
Tuberculosis (TB), caused by (), is a major public health issue in China. Nevertheless, the prevalence and drug resistance characteristics of isolates vary in different regions and provinces. In this study, we investigated the population structure, transmission dynamics and drug-resistant profiles of in Guangxi, located on the border of China.
METHODS
From February 2016 to April 2017, 462 clinical isolates were selected from 5 locations in Guangxi. Drug-susceptibility testing was performed using 6 common anti-tuberculosis drugs. The genotypic drug resistance and transmission dynamics were analyzed by the whole genome sequence.
RESULTS
Our data showed that the in Guangxi has high genetic diversity including Lineage 1 to Lineage 4, and mostly belong to Lineage 2 and Lineage 4. Novelty, 9.6% of Lineage 2 isolates were proto-Beijing genotype (L2.1), which is rare in China. About 12.6% of isolates were phylogenetically clustered and formed into 28 transmission clusters. We observed that the isolates with the high resistant rate of isoniazid (INH, 21.2%), followed by rifampicin (RIF, 13.2%), and 6.7%, 12.1%, 6.7% and 1.9% isolates were resistant to ethambutol (EMB), streptomycin (SM), ofloxacin (OFL) and kanamycin (KAN), respectively. Among these, 6.5% and 3.3% of isolates belong to MDR-TB and Pre-XDR, respectively, with a high drug-resistant burden. Genetic analysis identified the most frequently encountered mutations of INH, RIF, EMB, SM, OFL and KAN were _Ser315Thr (62.2%), _Ser450Leu (42.6%), _Met306Vol (45.2%), _Lys43Arg (53.6%), _Asp94Gly (29.0%) and _A1401G (66.7%), respectively. Additionally, we discovered that isolates from border cities are more likely to be drug-resistant than isolates from non-border cities.
CONCLUSION
Our findings provide a deep analysis of the genomic population characteristics and drug-resistant of in Guangxi, which could contribute to developing effective TB prevention and control strategies.
背景
由（）引起的结核病是中国的一个主要公共卫生问题。然而，不同地区和省份分离株的流行率和耐药特征各不相同。在本研究中，我们调查了位于中国边境的广西地区结核分枝杆菌的种群结构、传播动态和耐药谱。
方法
2016年2月至2017年4月，从广西5个地点选取462株临床结核分枝杆菌分离株。使用6种常见抗结核药物进行药敏试验。通过全基因组序列分析基因型耐药性和传播动态。
结果
我们的数据显示，广西的结核分枝杆菌具有高度的遗传多样性，包括谱系1至谱系4，且大多属于谱系2和谱系。新颖的是，9.6%的谱系2分离株为原始北京基因型（L2.1），这在中国较为罕见。约12.6%的分离株在系统发育上聚类并形成28个传播簇。我们观察到，异烟肼（INH）耐药率高的分离株（21.2%），其次是利福平（RIF，13.2%），12.1%、6.7%、6.7%和1.9%的分离株分别对乙胺丁醇（EMB）、链霉素（SM）、氧氟沙星（OFL）和卡那霉素（KAN）耐药。其中，分别有6.5%和3.3%的分离株属于耐多药结核病和广泛耐药前结核病，耐药负担较高。基因分析确定，INH、RIF、EMB、SM、OFL和KAN最常出现的突变分别为_Ser315Thr（62.2%）、_Ser450Leu（42.6%）、_Met306Vol（45.2%）、_Lys43Arg（53.6%）、_Asp94Gly（29.0%）和_A1401G（66.7%）。此外，我们发现边境城市的分离株比非边境城市的分离株更易耐药。
结论
我们的研究结果对广西结核分枝杆菌的基因组种群特征和耐药性进行了深入分析，这有助于制定有效的结核病预防和控制策略。
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