Sun Dongyuan, Song Ning, Li Minmin, Chen Xi, Zhang Xinyue, Yu Yang, Ying Jicheng, Xu Mengqi, Zheng Wentian, Han Chengbing, Ji Honghai, Jiang Yingying
School of Stomatology Weifang Medical University Weifang China.
Department of Stomatology Affiliated Hospital of Weifang Medical University Weifang China.
FASEB Bioadv. 2023 Jun 1;5(8):305-320. doi: 10.1096/fba.2023-00036. eCollection 2023 Aug.
N7-methylguanosine (m7G) modification is closely related to the occurrence of tumors. However, the m7G modification of circRNAs in oral squamous cell carcinoma (OSCC) remains to be investigated. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to measure the methylation levels of m7G and identify m7G sites in circRNAs in human OSCC and normal tissues. The host genes of differentially methylated and differentially expressed circRNAs were analyzed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and circRNA-miRNA-mRNA networks were predicted using the miRanda and miRDB databases. The analysis identified 2348 m7G peaks in 624 circRNAs in OSCC tissues. In addition, the source of m7G-methylated circRNAs in OSCC was mainly the sense overlap region compared with normal tissues. The most conserved m7G motif in OSCC tissues was CCUGU, whereas the most conserved motif in normal tissues was RCCUG (R = G/A). Importantly, GO enrichment and KEGG pathway analysis showed that the host genes of differentially methylated and differentially expressed circRNAs were involved in many cellular biological functions. Furthermore, the significantly differentially expressed circRNAs were analyzed to predict the circRNA-miRNA-mRNA networks. This study revealed the whole profile of circRNAs of differential m7G methylation in OSCC and suggests that m7G-modified circRNAs may impact the development of OSCC.
N7-甲基鸟苷（m7G）修饰与肿瘤的发生密切相关。然而，环状RNA（circRNA）在口腔鳞状细胞癌（OSCC）中的m7G修饰仍有待研究。采用甲基化RNA免疫沉淀测序（MeRIP-seq）技术检测人OSCC组织和正常组织中circRNA的m7G甲基化水平并鉴定m7G位点。通过基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析对差异甲基化和差异表达的circRNA的宿主基因进行分析，并使用miRanda和miRDB数据库预测circRNA- miRNA- mRNA网络。分析在OSCC组织的624个circRNA中鉴定出2348个m7G峰。此外，与正常组织相比，OSCC中m7G甲基化circRNA的来源主要是正义重叠区域。OSCC组织中最保守的m7G基序是CCUGU，而正常组织中最保守的基序是RCCUG（R = G/A）。重要的是，GO富集分析和KEGG通路分析表明，差异甲基化和差异表达的circRNA的宿主基因参与许多细胞生物学功能。此外，对显著差异表达的circRNA进行分析以预测circRNA- miRNA- mRNA网络。本研究揭示了OSCC中差异m7G甲基化的circRNA全貌，并表明m7G修饰的circRNA可能影响OSCC的发展。
