Hayunga E G, Sumner M P
J Parasitol. 1986 Apr;72(2):283-91.
Adult Schistosoma mansoni were radiolabeled by direct radioiodination using the Bolton-Hunter reagent or by metabolic labeling using radioactive hexose precursors. Tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent Nonidet P-40, then applied to chromatography columns containing the following immobilized lectins: Con A, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from Ricinus communis and Helix pomatia. SDS-PAGE analysis of the sugar eluates from these columns revealed the presence of 15 glycoproteins with apparent molecular weights greater than or equal to 300,000, 215,000, 168,000, 152,000, 134,000, 122,000, 108,000, 83,000, 58,000, 53,000, 46,000, 41,000, 34,000, 30,000 and 23,500. Many of the glycoproteins reacted with more than one lectin. Information about carbohydrate content and lectin binding provides a preliminary characterization of the tegumental glycoprotein antigens of adult worms.
成年曼氏血吸虫通过使用博尔顿-亨特试剂进行直接放射性碘化或使用放射性己糖前体进行代谢标记来进行放射性标记。通过冻融或在非离子去污剂Nonidet P-40中孵育来提取皮层物质，然后将其应用于含有以下固定化凝集素的色谱柱：刀豆球蛋白A、扁豆凝集素、麦胚凝集素、大豆凝集素以及蓖麻和苹果螺的凝集素。对这些色谱柱的糖洗脱液进行SDS-PAGE分析，结果显示存在15种糖蛋白，其表观分子量分别大于或等于300,000、215,000、168,000、152,000、134,000、122,000、108,000、83,000、,58,000、53,000、46,000、41,000、34,000、30,000和23,500。许多糖蛋白与不止一种凝集素发生反应。有关碳水化合物含量和凝集素结合的信息为成年虫皮层糖蛋白抗原提供了初步特征描述。
