SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy.
Center for Life Nano and Neuro Science, Istituto Italiano di Tecnologia (IIT), Viale Regina Elena 291, 00161 Rome, Italy.
Analyst. 2023 Sep 11;148(18):4429-4437. doi: 10.1039/d3an00804e.
We report on the use of biochips based on one-dimensional photonic crystals sustaining Bloch surface waves to specifically detect target miRNA that is characteristic of hemorrhagic stroke (miR-16-5p) at low concentration in a buffer solution. The biochips were functionalized with streptavidin and ssDNA oligonucleotides to enable miRNA detection. To discriminate the target miRNA from a non-specific control (miR-101a-3p), we made use of an optical platform developed to work both in label-free and fluorescence detection modes. We demonstrate that the limit of detection provided when operating in the fluorescence mode allows us to specifically detect the target miRNA down to 1 ng mL (140 pM), which matches the recommendations for diagnostic miRNA assays, 5 ng mL. The low costs open the way towards the application of these disposable optical biochips based on 1DPC sustaining Bloch surface waves as a promising tool for early disease detection in a liquid biopsy format.
我们报告了使用基于一维光子晶体支持布洛赫表面波的生物芯片来特异性检测低浓度缓冲溶液中出血性中风特征的靶 miRNA（miR-16-5p）的方法。生物芯片通过链霉亲和素和 ssDNA 寡核苷酸进行功能化，以实现 miRNA 的检测。为了从非特异性对照（miR-101a-3p）中区分靶 miRNA，我们利用开发的光学平台在无标记和荧光检测模式下工作。我们证明，在荧光模式下操作时提供的检测限允许我们特异性地检测到低至 1ng mL（140pM）的靶 miRNA，这与诊断 miRNA 测定的建议值 5ng mL 相匹配。低成本为基于一维光子晶体支持布洛赫表面波的一次性光学生物芯片的应用开辟了道路，这种生物芯片作为液体活检格式中早期疾病检测的有前途的工具。
