Lewis R W, Harwood J L, Richards R J
Biochem J. 1986 Apr 1;235(1):75-9. doi: 10.1042/bj2350075.
A method is described for the preparation of rat pulmonary surfactant, radiolabelled specifically in the phosphatidylcholine species, which may be used for degradative studies of the lipoprotein complex. Intravenously administered [methyl-14C]choline chloride is maximally incorporated into alveolar surface surfactant 8 h after injection, and more than 97% of this radiolabel is present in the phosphatidylcholine fraction of the surfactant and, of this, 75% is associated with the dipalmitoyl phosphatidylcholine species. Electron microscopy indicates that the isolated surfactant has a similar physical form to that found at the alveolar surface. The mineral alpha-quartz can be used to increase the yield of surfactant lavaged from the lung surface, but the complex isolated from rats treated in this manner has a low specific radioactivity (less than 1000 d.p.m./mg) compared with that prepared from control animals (22860 d.p.m./mg).
本文描述了一种制备大鼠肺表面活性剂的方法，该表面活性剂在磷脂酰胆碱种类中被特异性放射性标记，可用于脂蛋白复合物的降解研究。静脉注射的[甲基-14C]氯化胆碱在注射后8小时最大程度地掺入肺泡表面活性剂中，并且这种放射性标记的97%以上存在于表面活性剂的磷脂酰胆碱部分中，其中75%与二棕榈酰磷脂酰胆碱种类相关。电子显微镜显示，分离出的表面活性剂具有与肺泡表面发现的类似物理形态。矿物α-石英可用于提高从肺表面灌洗的表面活性剂产量，但与从对照动物制备的表面活性剂相比，以这种方式处理的大鼠分离出的复合物具有较低的比放射性（小于1000 d.p.m./mg）（22860 d.p.m./mg）。
