Glatz T, Ikegami M, Jobe A
Pediatr Res. 1982 Sep;16(9):711-5. doi: 10.1203/00006450-198209000-00002.
[3H]-Palmitate labeled natural lamb surfactant and free [14C]-choline were mixed with the lung fluid of 11 term lambs at cesarean section, before the first breath. After receiving the isotope, the lambs were delivered, allowed to breathe spontaneously, and were subsequently sacrificed from 5 min to 96 h of age. Alveolar washes, lung homogenates, microsomal and lamellar body fractions of lungs, and pulmonary alveolar macrophages were examined for the presence of labeled phosphatidylcholine. Analysis of the labeled natural surfactant kinetic data revealed an apparent t 1/2 of phosphatidylcholine in the whole lung of 6.0 days. This half-life can be interpreted only as a rough estimate. Appearance of considerable [3H] labeled phosphatidylcholine in the lung homogenates demonstrated uptake of phosphatidylcholine from alveoli into lung tissue. The surfactant-associated label in homogenates was localized preferentially to lamellar body fractions. Some of the administered [14C]-choline appeared in phosphatidylcholine. Almost all of this labeled phosphatidylcholine was associated with the homogenate. Extremely small % of administered [3H] and [14C] were found in pulmonary alveolar macrophages.
将[3H] - 棕榈酸盐标记的天然羊肺表面活性物质和游离的[14C] - 胆碱在剖宫产时、第一次呼吸之前与11只足月羔羊的肺液混合。接受同位素后，羔羊出生，使其自主呼吸，随后在出生后5分钟至96小时处死。检查肺泡灌洗液、肺匀浆、肺的微粒体和板层小体组分以及肺泡巨噬细胞中是否存在标记的磷脂酰胆碱。对标记的天然表面活性物质动力学数据的分析显示，整个肺中磷脂酰胆碱的表观半衰期为6.0天。这个半衰期只能作为一个粗略的估计。肺匀浆中出现大量[3H]标记的磷脂酰胆碱表明磷脂酰胆碱从肺泡摄取到肺组织中。匀浆中与表面活性物质相关的标记优先定位于板层小体组分。一些给予的[14C] - 胆碱出现在磷脂酰胆碱中。几乎所有这种标记的磷脂酰胆碱都与匀浆相关。在肺泡巨噬细胞中发现给予的[3H]和[14C]的比例极低。
