Castellani W J, Van Lente F, Chou D
Clin Chem. 1986 Sep;32(9):1672-6.
A general-purpose bench-top laboratory robot was programmed to perform sample preparation for immunoprecipitative and immunoinhibitory determinations of cardiac isoenzymes with commercial kits. Run size could be varied from 1 to 24 patients' samples, and was determined by the robot without prompting. The robot placed the processed samples into a sample rotor for dried transfer to a batch centrifugal analyzer. Robotic precision compared well with that of manual performance of the same procedures at four concentrations of lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 activity and at one of two concentrations of creatine kinase (EC 2.7.3.2.) isoenzyme MB activity; for the other creatine kinase MB concentration, use of the robot significantly improved precision. Results for 100 samples from patients after open-heart surgery were highly correlated with manually obtained results for both isoenzyme determinations (r less that 0.96 each). Time to completion for small run sizes for either approach was comparable about 25 min. For larger batches, robotic run time increased rapidly, to 169 min for 24 patients' samples vs 41 min by manual assay.
一台通用台式实验室机器人被编程，使用商业试剂盒对心脏同工酶进行免疫沉淀和免疫抑制测定的样本制备。运行样本数量可在1至24个患者样本之间变化，由机器人自动确定，无需提示。机器人将处理后的样本放入样本转子中，以便干燥转移至批量离心分析仪。在乳酸脱氢酶（EC 1.1.1.27）同工酶1活性的四个浓度以及肌酸激酶（EC 2.7.3.2.）同工酶MB活性的两个浓度之一的情况下，机器人的精度与相同操作的手动执行情况相当；对于另一个肌酸激酶MB浓度，使用机器人显著提高了精度。来自心脏直视手术后患者的100个样本的结果与两种同工酶测定的手动获得结果高度相关（每种相关系数r均小于0.96）。对于两种方法，小样本量运行的完成时间相当，约为25分钟。对于较大批量，机器人运行时间迅速增加，24个患者样本的运行时间为169分钟，而手动测定为41分钟。
