在通过免疫化学方法去除其他同工酶后，使用离心分析仪检测乳酸脱氢酶同工酶1。
Lactate dehydrogenase isoenzyme 1 with the centrifugal analyzer after immunochemical removal of other isoenzymes.
作者信息
Bruns D E, Emerson J C, Bertholf R L, Hill K E, Savory J
出版信息
Clin Chem. 1981 Jun;27(6):922-3.
We describe a centrifugal analyzer method for measuring the LD-1 isoenzyme of lactate dehydrogenase (EC 1.1.1.27) in serum, after immunochemical precipitation of the other four isoenzymes. Enzymic activity was measured kinetically at 30 degrees C with the pyruvate-to-lactate assay. The method for LD-1 was linear to 1000 U/L. The precision (CV) of the assay was 1.0--2.2% within-run and 3.1--4.5% day-to-day. The reference interval was 26--73 U/L (n = 51), corresponding to 21--35% of total LD activity.
我们描述了一种离心分析仪方法，用于在对其他四种同工酶进行免疫化学沉淀后，测定血清中乳酸脱氢酶（EC 1.1.1.27）的LD-1同工酶。采用丙酮酸到乳酸的检测方法，在30℃下动态测定酶活性。LD-1的检测方法在1000 U/L范围内呈线性。该检测方法的批内精密度（CV）为1.0%-2.2%，日间精密度为3.1%-4.5%。参考区间为26-73 U/L（n = 51），相当于总LD活性的21%-35%。
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