Stephenson Institute of Renewable Energy, University of Liverpool, Liverpool L69 7ZF, U.K.
Department of Physics, University of Liverpool, Liverpool L69 7ZE, U.K.
J Phys Chem B. 2023 Aug 24;127(33):7283-7290. doi: 10.1021/acs.jpcb.3c04466. Epub 2023 Aug 9.
Elucidating the photosynthetic processes that occur within the reaction center-light-harvesting 1 (RC-LH1) supercomplexes from purple bacteria is crucial for uncovering the assembly and functional mechanisms of natural photosynthetic systems and underpinning the development of artificial photosynthesis. Here, we examined excitation energy transfer of various RC-LH1 supercomplexes of using transient absorption spectroscopy, coupled with lifetime density analysis, and studied the roles of the integral transmembrane polypeptides, PufX and PufY, in energy transfer within the RC-LH1 core complex. Our results show that the absence of PufX increases both the LH1 → RC excitation energy transfer lifetime and distribution due to the role of PufX in defining the interaction and orientation of the RC within the LH1 ring. While the absence of PufY leads to the conformational shift of several LH1 subunits toward the RC, it does not result in a marked change in the excitation energy transfer lifetime.
阐明在紫色细菌的反应中心-捕光 1 （RC-LH1）超复合体中发生的光合作用过程对于揭示自然光合作用系统的组装和功能机制以及支持人工光合作用的发展至关重要。在这里，我们使用瞬态吸收光谱结合寿命密度分析研究了各种 RC-LH1 超复合体的激发能量转移，并研究了整合跨膜多肽 PufX 和 PufY 在 RC-LH1 核心复合物内能量转移中的作用。我们的结果表明，PufX 的缺失会增加 LH1→RC 激发能量转移寿命和分布，因为 PufX 在确定 RC 在 LH1 环中的相互作用和取向方面发挥作用。虽然 PufY 的缺失会导致几个 LH1 亚基向 RC 发生构象转变，但它不会导致激发能量转移寿命的明显变化。
