Adhab Mustafa, Zhang Yu, Schoelz James
Division of Plant Science and Technology, University of Missouri, Columbia, MO, 65211, USA.
Virology. 2023 Oct;587:109854. doi: 10.1016/j.virol.2023.109854. Epub 2023 Aug 1.
Over the past decades, several studies have examined the subcellular localization of the cauliflower mosaic virus (CaMV) P6 protein by tagging it with GFP (P6-GFP). These investigations have been essential in the development of models for inclusion body formation, nuclear transport, and microfilament-associated intracellular movement of P6 inclusion bodies for delivery of virions to plasmodesmata. Although it was shown early on that the translational transactivation function of P6-GFP was comparable to wild type P6, it has not been possible to incorporate a P6-GFP gene into an infectious clone of CaMV. Consequently, it has not been possible to formally prove that a P6-GFP fusion is comparable in function to the unmodified P6 protein. Here we show that transient expression of P6-GFP can complement a defective CaMV replicon through gene expression, replication and encapsidation, which validates the relevance of P6-GFP subcellular localization studies for understanding the development of CaMV infections.
在过去几十年里，多项研究通过用绿色荧光蛋白（P6-GFP）标记花椰菜花叶病毒（CaMV）的P6蛋白，对其亚细胞定位进行了研究。这些研究对于构建包含体形成、核运输以及P6包含体与微丝相关的细胞内运动模型以将病毒粒子递送至胞间连丝至关重要。尽管早期研究表明P6-GFP的翻译反式激活功能与野生型P6相当，但一直无法将P6-GFP基因整合到CaMV的感染性克隆中。因此，尚未能正式证明P6-GFP融合蛋白在功能上与未修饰的P6蛋白相当。在此我们表明，P6-GFP的瞬时表达可通过基因表达、复制和衣壳化来补充有缺陷的CaMV复制子，这证实了P6-GFP亚细胞定位研究对于理解CaMV感染发展的相关性。
