使用合成寡脱氧核苷酸分离α1型IV型胶原cDNA克隆。
Isolation of an alpha 1 type-IV collagen cDNA clone using a synthetic oligodeoxynucleotide.
作者信息
Nath P, Laurent M, Horn E, Sobel M E, Zon G, Vogeli G
出版信息
Gene. 1986;43(3):301-4. doi: 10.1016/0378-1119(86)90220-9.
We have isolated a cDNA clone (pCIV-1-225) for the alpha 1 subunit of basement membrane (type IV) collagen from a cDNA library made from Engelbreth-Holm-Swarm mouse tumor RNA. The cDNA library was screened with synthetic oligodeoxynucleotides derived from published amino acid (aa) sequences (Schuppan et al., 1982). Nucleotide sequence data established the identity of our cDNA clone to encode an alpha 1 type IV collagen. This clone contains 270 aa of the helical region and has three interruptions in the Gly-X-Y repeat unit.
我们从Engelbreth-Holm-Swarm小鼠肿瘤RNA构建的cDNA文库中分离出了基底膜（IV型）胶原α1亚基的一个cDNA克隆（pCIV-1-225）。用从已发表的氨基酸（aa）序列（Schuppan等人，1982年）推导而来的合成寡脱氧核苷酸对该cDNA文库进行筛选。核苷酸序列数据确定我们的cDNA克隆编码α1型IV胶原。该克隆包含螺旋区的270个氨基酸，并且在Gly-X-Y重复单元中有三处中断。
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