Center for Advanced Research and Innovation, Office of the Vice President for Research and Innovation, Angeles University Foundation, Pampanga 2009 Philippines; College of Allied Medical Professions, Angeles University Foundation, Pampanga 2009 Philippines.
College of Arts and Sciences, Angeles University Foundation, Pampanga 2009 Philippines.
J Chromatogr A. 2023 Sep 13;1706:464228. doi: 10.1016/j.chroma.2023.464228. Epub 2023 Jul 20.
The culturable endophytic bacteria from the weeds Cleome rutidosperma of the family Cleomaceae and Digitaria sanguinalis of the family Poaceae obtained from a previous dumpsite in Pampanga, Philippines have been assessed for their anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and the analytes with such activity should be identified. However, due to the limited amounts collected from the isolation process, 1.8 mg yield of compound 1 from the endophyte of C. rutidosperma and 1.2 mg of a mixture from the endophyte of D. sanguinalis were selected for LC-MS analysis. The production of compounds from the culturable endophytic bacteria Pseudomonas aeruginosa- determined by gene-sequencing, an untargeted and data-independent analysis (DIA) by ultra-high performance liquid chromatography-high resolution-elevated energy mass spectrometry (UHPLC-HR-MS) technique was employed to profile the metabolites present in the two high-performance liquid chromatography (HPLC) fractions. The analytes present from P. aeruginosa detected by UHPLC-HR-MS isolated from C. rutidosperma was phenazine-1-carboxylic acid (1), and for D. sanguinalis were chamigrenal (2), dialkyl resorcinol (3), and a pyoverdine elicitor (4). This study proves that UHPLC-HR-MS could identify the anti-MRSA constituents in P. aeruginosa from commensal weeds C. rutidosperma and D. sanguinalis. The UHPLC-HR-MS could help strengthen metabolomics antibacterial research and its related applications from a future perspective. Application of metabolomics research using UHPLC-HR-MS could enhance the rehabilitation of dumpsites by the microbial community present.
从菲律宾邦板牙省先前的垃圾填埋场获得的 Cleome rutidosperma（山柑科）和 Digitaria sanguinalis（禾本科）杂草中的可培养内生细菌已被评估其抗耐甲氧西林金黄色葡萄球菌（MRSA）活性，并且应该鉴定具有这种活性的分析物。然而，由于从分离过程中收集的数量有限，从内生菌 C. rutidosperma 中选择了 1.8mg 的化合物 1 和从内生菌 D. sanguinalis 中选择了 1.2mg 的混合物进行 LC-MS 分析。通过基因测序确定了可培养内生细菌铜绿假单胞菌的化合物产生情况，通过超高效液相色谱-高分辨率-高能量质谱 (UHPLC-HR-MS) 技术进行非靶向和数据独立分析 (DIA)，以分析两种高效液相色谱 (HPLC) 馏分中存在的代谢产物。通过 UHPLC-HR-MS 从 C. rutidosperma 中分离出的、来自铜绿假单胞菌的分析物为吩嗪-1-羧酸（1），而来自 D. sanguinalis 的分析物为香豆素醛（2）、二烷基间苯二酚（3）和一种吡咯并紫红素诱导剂（4）。这项研究证明 UHPLC-HR-MS 可以鉴定来自共生杂草 C. rutidosperma 和 D. sanguinalis 的铜绿假单胞菌中的抗 MRSA 成分。从未来的角度来看，UHPLC-HR-MS 可用于鉴定和识别微生物群落中存在的代谢产物，这有助于加强代谢组学抗菌研究及其相关应用。使用 UHPLC-HR-MS 的代谢组学研究的应用可以增强微生物群落对垃圾填埋场的修复。
