Gallily R, Stain I, Zaady O
Immunol Lett. 1986 Sep;13(3):151-8. doi: 10.1016/0165-2478(86)90048-9.
Pretreatment of murine bone marrow-derived macrophages with wheat germ agglutinin (WGA) and concanavalin A (Con A) markedly enhanced the uptake of Staphylococcus aureus H and Escherichia coli 08, respectively. Pretreatment of the respective bacteria with these lectins also greatly increased their uptake by the macrophages. An increase of up to 40-fold in uptake of E. coli 08 by the macrophages was observed following their pretreatment with Con A in the absence of serum and at a low temperature. On the other hand, pretreatment of macrophages with either WGA or Con A suppressed very markedly or abolished completely their bactericidal activity against S. aureus H and E. coli 08, respectively. As macrophage chemiluminescence reactions to zymosan were also very greatly suppressed following pretreatment with WGA and Con A, we suggest that these lectins suppressed macrophage bactericidal activity by inhibiting reactions involving chemiluminescence.
用麦胚凝集素（WGA）和伴刀豆球蛋白A（Con A）对小鼠骨髓来源的巨噬细胞进行预处理，可分别显著增强其对金黄色葡萄球菌H和大肠杆菌O8的摄取。用这些凝集素对相应细菌进行预处理，也会大大增加巨噬细胞对它们的摄取。在无血清且低温条件下，用Con A对巨噬细胞进行预处理后，观察到巨噬细胞对大肠杆菌O8的摄取增加了40倍。另一方面，用WGA或Con A对巨噬细胞进行预处理，分别非常显著地抑制或完全消除了它们对金黄色葡萄球菌H和大肠杆菌O8的杀菌活性。由于用WGA和Con A预处理后，巨噬细胞对酵母聚糖的化学发光反应也受到极大抑制，我们认为这些凝集素通过抑制涉及化学发光的反应来抑制巨噬细胞的杀菌活性。
