Gallily R, Vray B, Stain I, Sharon N
Immunology. 1984 Aug;52(4):679-86.
Exposure of thioglycollate-elicited murine peritoneal macrophages to wheat germ agglutinin (WGA) increased markedly the uptake of six different bacteria, which have surface receptors for the lectin. Uptake of Staphylococcus aureus H was higher by 3-5-fold, of S. aureus 52A2 by 1.8-fold, of S. aureus 52A5 by 1.7-fold, of S. albus by 2.3-fold, of Shigella flexneri by 6-fold and of Micrococcus luteus by 6.5-fold. Klebsiella pneumoniae, devoid of receptors for WGA, was not phagocytosed following pretreatment of macrophages with the lectin. Pretreatment of the bacteria with the lectin also resulted, in most cases, in an increase in phagocytosis. Interaction of WGA with the macrophages and with the bacteria, as well as the potentiation of phagocytosis, was abolished by tri-N-acetylchitotriose, a saccharide that binds specifically to WGA, but not by monosaccharides which do not interact with this lectin. With non-elicited macrophages, enhancement of phagocytosis by WGA was less pronounced, probably because of the higher number of lectin-binding sites (5-fold) on the elicited cells. Peanut agglutinin and soybean agglutinin, that bind to macrophages but not to the bacteria studied, lack the ability to potentiate phagocytosis. Macrophage surface sugars thus appear to play an important role in phagocytosis by serving as receptors for lectins that form bridges between the macrophages and the microorganisms.
用巯基乙酸盐诱导的小鼠腹腔巨噬细胞暴露于麦胚凝集素（WGA）后，对六种不同细菌的摄取显著增加，这些细菌具有该凝集素的表面受体。金黄色葡萄球菌H的摄取量提高了3至5倍，金黄色葡萄球菌52A2提高了1.8倍，金黄色葡萄球菌52A5提高了1.7倍，白色链霉菌提高了2.3倍，弗氏志贺菌提高了6倍，藤黄微球菌提高了6.5倍。缺乏WGA受体的肺炎克雷伯菌在用该凝集素预处理巨噬细胞后未被吞噬。用凝集素预处理细菌在大多数情况下也会导致吞噬作用增加。三 - N - 乙酰壳三糖（一种与WGA特异性结合的糖类）可消除WGA与巨噬细胞和细菌的相互作用以及吞噬作用的增强，但不与该凝集素相互作用的单糖则不能。对于未诱导的巨噬细胞，WGA对吞噬作用的增强作用不太明显，这可能是因为诱导细胞上凝集素结合位点的数量更多（5倍）。与巨噬细胞结合但不与所研究细菌结合的花生凝集素和大豆凝集素缺乏增强吞噬作用的能力。因此，巨噬细胞表面糖似乎在吞噬作用中起着重要作用，它们作为凝集素的受体，在巨噬细胞和微生物之间形成桥梁。
