Narayanan R B, Girdhar B K, Desikan K V
Int J Lepr Other Mycobact Dis. 1986 Sep;54(3):423-6.
A study was made on the Langerhans' cells at the sites of contact sensitivity skin reactions in 45 untreated leprosy patients. The skin reaction was induced by 2,4-dinitrochlorobenzene (DNCB). Langerhans' cells were quantitated using OKT6 monoclonal antibody and indirect immunofluorescence. Clinically, the skin reaction in the tuberculoid patients was positive at 4, 24, and 48 hr, while the lepromatous patients failed to respond at any of the time intervals. Sequential histological analysis of the skin reaction showed predominantly mononuclear cell infiltrates around the blood vessels and neurovascular bundles in both the tuberculoid and lepromatous patients. Time kinetic assessment showed no difference in the numbers and distribution of OKT6+ epidermal Langerhans' cells at the site of the DNCB skin reactions among the tuberculoid and lepromatous patients. This, therefore, suggests that either there is a functional defect in Langerhans' cells or some other mechanism(s) such as a T-cell abnormality is responsible for the lack of clinical reaction in lepromatous patients.
对45例未经治疗的麻风患者接触性敏感皮肤反应部位的朗格汉斯细胞进行了研究。皮肤反应由2,4-二硝基氯苯（DNCB）诱发。使用OKT6单克隆抗体和间接免疫荧光对朗格汉斯细胞进行定量。临床上，结核样型患者的皮肤反应在4小时、24小时和48小时呈阳性，而瘤型患者在任何时间间隔均无反应。对皮肤反应进行的连续组织学分析显示，结核样型和瘤型患者的血管和神经血管束周围主要为单核细胞浸润。时间动力学评估显示，结核样型和瘤型患者DNCB皮肤反应部位OKT6 + 表皮朗格汉斯细胞的数量和分布没有差异。因此，这表明要么朗格汉斯细胞存在功能缺陷，要么是其他一些机制，如T细胞异常，导致瘤型患者缺乏临床反应。
