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麻疹病毒第六(L)基因克隆的鉴定及转录图谱

Characterization of clones for the sixth (L) gene and a transcriptional map for morbilliviruses.

作者信息

Rima B K, Baczko K, Clarke D K, Curran M D, Martin S J, Billeter M A, ter Meulen V

出版信息

J Gen Virol. 1986 Sep;67 ( Pt 9):1971-8. doi: 10.1099/0022-1317-67-9-1971.

DOI:10.1099/0022-1317-67-9-1971
PMID:3755751
Abstract

cDNA clones of the largest RNA transcript of the canine distemper and measles morbilliviruses were characterized. This presumably codes for the L protein of these viruses. mRNA 4 was identified as coding for the haemagglutinin protein of measles virus. From an analysis of readthrough transcripts representing tandem copies of two or three genes we established a transcriptional map and the gene order on the negative strand genome of the morbilliviruses to be 3'-N-P + C-M-F-H-L-5'. The data exclude the presence of small intervening genes between the six major genes of morbilliviruses and indicate the gene order to be similar to that of Sendai virus and different from that of simian virus 5.

摘要

犬瘟热病毒和麻疹病毒最大RNA转录本的cDNA克隆得到了鉴定。这可能编码这些病毒的L蛋白。mRNA 4被鉴定为编码麻疹病毒的血凝素蛋白。通过对代表两个或三个基因串联拷贝的通读转录本进行分析,我们建立了麻疹病毒负链基因组的转录图谱和基因顺序为3'-N-P+C-M-F-H-L-5'。这些数据排除了麻疹病毒六个主要基因之间存在小间隔基因的可能性,并表明基因顺序与仙台病毒相似,与猿猴病毒5不同。

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J Gen Virol. 1986 Sep;67 ( Pt 9):1971-8. doi: 10.1099/0022-1317-67-9-1971.
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J Virol. 1994 Mar;68(3):1532-43. doi: 10.1128/JVI.68.3.1532-1543.1994.
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Characterization of in vitro transcription and transcriptional products of measles virus.麻疹病毒的体外转录及转录产物的特性分析
J Virol. 1987 Nov;61(11):3381-7. doi: 10.1128/JVI.61.11.3381-3387.1987.
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Altered transcription of a defective measles virus genome derived from a diseased human brain.源自患病人类大脑的缺陷型麻疹病毒基因组转录的改变。
EMBO J. 1987 Mar;6(3):681-8. doi: 10.1002/j.1460-2075.1987.tb04808.x.
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