Selvarajan Ethiraj
Department of Genetic Engineering, School of Bioengineering, Faculty of Engineering and Technology, College of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur 603203, India..
Int J Biol Macromol. 2023 Oct 1;250:126138. doi: 10.1016/j.ijbiomac.2023.126138. Epub 2023 Aug 7.
The conversion of lignocellulosic waste to energy offers a cost-effective biofuel. The current study discusses the utilization of cellulose in rice husks by lichen-associated Streptomyces sp. MS2A via carbohydrate metabolism. Out of 39 actinobacteria, one actinobacterial strain MS2A, showed CMCase, FPase, and cellobiohydrolase activity. The whole genome analysis of Streptomyces sp. MS2A showed maximum similarity with Streptomyces sp. CCM_MD2014. The genome analysis confirmed the presence of cellulose-degrading genes along with xylan-degrading genes that code for GH3, GH6, GH9, GH11, GH43, GH51, and 15 other GH families with glycosyl transferase, carbohydrate-binding modules, and energy metabolism groups. Protein family analysis corroborates the enzyme family. Among the 19,402 genes of Streptomyces sp. MS2A, approximately 70 GH family codes for lignocellulose degradation enzymes. The structure of cellulase was modeled and validated. Scanning electron microscopy and gas chromatography-mass spectrometry (GCMS) was performed to analyze the lignocellulosic degradation of rice husk and the end product bioethanol.
将木质纤维素废料转化为能源可提供一种经济高效的生物燃料。当前的研究讨论了地衣相关链霉菌属MS2A通过碳水化合物代谢对稻壳中纤维素的利用。在39株放线菌中，一株放线菌菌株MS2A表现出羧甲基纤维素酶（CMCase）、滤纸酶（FPase）和纤维二糖水解酶活性。链霉菌属MS2A的全基因组分析显示与链霉菌属CCM_MD2014具有最大相似性。基因组分析证实存在纤维素降解基因以及木聚糖降解基因，这些基因编码GH3、GH6、GH9、GH11、GH43、GH51以及其他15个具有糖基转移酶、碳水化合物结合模块和能量代谢组的GH家族。蛋白质家族分析证实了酶家族。在链霉菌属MS2A的19402个基因中，约70个GH家族编码木质纤维素降解酶。对纤维素酶的结构进行了建模和验证。进行了扫描电子显微镜和气相色谱 - 质谱联用（GCMS）分析，以研究稻壳的木质纤维素降解情况及最终产物生物乙醇。
