East China University of Science and Technology, People's Republic of China.
East China University of Science and Technology, People's Republic of China.
Int J Biol Macromol. 2023 Oct 1;250:126172. doi: 10.1016/j.ijbiomac.2023.126172. Epub 2023 Aug 7.
Obesity has emerged as a crucial factor impacting people's lives, and gut microbiota disorders contribute to its development and progression. Auricularia auricula-judae (Bull.) polysaccharides (AAPs), a traditional functional food in Asia, exhibit potential anti-obesity effects. However, the specific mechanism still needs to be further confirmed. This study investigated the beneficial effects and specific mechanisms of AAPs on obesity. Firstly, AAPs showed significant improvements in overweight, insulin resistance, glucose and lipid metabolism disorders, and liver damage in obese mice. Additionally, AAPs ameliorated gut microbiota disorders, promoting the proliferation of beneficial bacteria like Lactobacillus and Roseburia, resulting in increased levels of SCFAs, folate, and cobalamin. Simultaneously, AAPs inhibited the growth of harmful bacteria, thereby protecting intestinal barrier function, improving endotoxemia, and decreasing the levels of inflammatory factors such as TNF-α and IL-6. Furthermore, AAPs can inhibit the TLR4/JNK signaling pathway while promoting the activation of AKT and AMPK. Importantly, our study underscored the pivotal role of gut microbiota in the anti-obesity effects of AAPs, as evidenced by fecal microbiota transplantation experiments. In conclusion, our findings elucidated that AAPs improve obesity by regulating gut microbiota and TLR4/JNK signaling pathway, offering novel perspectives for further conclusion the anti-obesity potential of AAPs.
肥胖已成为影响人们生活的一个关键因素，而肠道微生物群紊乱是导致肥胖发生和发展的因素之一。黑木耳多糖（AAPs）是亚洲传统的功能性食品，具有潜在的抗肥胖作用。然而，其具体机制仍需进一步证实。本研究探讨了 AAPs 对肥胖的有益作用及具体作用机制。首先，AAPs 可显著改善肥胖小鼠的超重、胰岛素抵抗、糖脂代谢紊乱和肝损伤。此外，AAPs 可改善肠道微生物群紊乱，促进有益菌如乳酸杆菌和罗斯伯里氏菌的增殖，从而增加 SCFAs、叶酸和钴胺素的水平。同时，AAPs 抑制有害菌的生长，从而保护肠道屏障功能，改善内毒素血症，并降低 TNF-α和 IL-6 等炎症因子的水平。此外，AAPs 可抑制 TLR4/JNK 信号通路，同时促进 AKT 和 AMPK 的激活。重要的是，我们的研究强调了肠道微生物群在 AAPs 抗肥胖作用中的关键作用，粪便微生物群移植实验为此提供了证据。总之，我们的研究结果表明，AAPs 通过调节肠道微生物群和 TLR4/JNK 信号通路来改善肥胖，为进一步研究 AAPs 的抗肥胖潜力提供了新的视角。
